Rabbit Polyclonal IkB-b(Phospho-Ser23) Antibody

The IkB-β (Phospho-Ser23) antibody is a specialized tool used to detect the phosphorylated form of Inhibitor of kappa B-beta (IkB-β) at serine residue 23. IkB-β is a member of the IkB family, which regulates the NF-κB signaling pathway by binding to and sequestering NF-κB transcription factors in the cytoplasm. Upon cellular stimulation (e.g., cytokines, pathogens, or stress), IkB proteins undergo phosphorylation, ubiquitination, and proteasomal degradation, allowing NF-κB to translocate to the nucleus and activate target genes involved in inflammation, immunity, and cell survival. Phosphorylation at Ser23 is a critical post-translational modification that primes IkB-β for degradation, thereby modulating the duration and intensity of NF-κB signaling. This phosphorylation event is typically mediated by the IκB kinase (IKK) complex, particularly IKKβ. The IkB-β (Phospho-Ser23) antibody enables researchers to study this regulatory step in diverse contexts, including inflammatory diseases, cancer, and immune responses. It is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation status, pathway activation, or drug effects. Specificity validation often involves comparing phosphorylated vs. non-phosphorylated IkB-β or using cells treated with pathway activators/inhibitors. Understanding IkB-β phosphorylation dynamics aids in deciphering NF-κB-related mechanisms and therapeutic targeting.