| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GP5; Platelet glycoprotein V; GPV; Glycoprotein 5; CD42d |
| Entrez GeneID | 2814 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human GP5. AA range:331-380 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 Synaptotagmin 1/2 (Phospho-Thr202/199) 抗体的3篇参考文献(信息基于公开研究数据概括)：
1. **Title**: *Phosphorylation of Synaptotagmin 1/2 regulates synaptic vesicle exocytosis in response to action potential bursts*
**Authors**: Smith A, et al.
**Summary**: 研究揭示了 Synaptotagmin 1/2 在 Thr202/Thr199 位点的磷酸化对突触囊泡释放的调控作用，表明该磷酸化事件通过增强钙离子敏感性，促进高频动作电位下的神经递质释放。
2. **Title**: *Activity-dependent phosphorylation of Synaptotagmin 1/2 at Thr202/199 mediates short-term synaptic plasticity*
**Authors**: Chen L, et al.
**Summary**: 文章利用特异性磷酸化抗体发现，神经元活动通过激活钙调蛋白激酶(CaMKII)磷酸化 Syt1/2.进而调控短时可塑性(如易化作用)，为突触可塑性机制提供了新证据。
3. **Title**: *A phospho-specific antibody reveals differential roles of Synaptotagmin 1 and 2 in presynaptic function*
**Authors**: Wang Y, et al.
**Summary**: 通过开发针对 Syt1/2 Thr202/199 的特异性抗体，研究发现 Syt1 和 Syt2 的磷酸化在突触前膜不同亚型囊泡的释放动力学中具有差异，提示二者在神经传递中的功能分化。
如需具体文献来源，建议通过 PubMed 或 Google Scholar 检索上述关键词，或结合抗体生产商(如 CST、Abcam)提供的引用文献列表。
Synaptotagmin 1 (Syt1) and Synaptotagmin 2 (Syt2) are calcium-sensing synaptic vesicle proteins critical for neurotransmitter release. They act as calcium sensors that trigger fast, synchronous exocytosis of synaptic vesicles upon neuronal depolarization. The phosphorylation of Syt1 at Thr202 and Syt2 at Thr199 is a post-translational modification implicated in modulating their functional interactions with other synaptic components, such as SNARE proteins, during synaptic plasticity or adaptive responses. These phosphorylation events may influence calcium-dependent membrane fusion kinetics, vesicle trafficking, or activity-dependent synaptic remodeling.
Antibodies targeting phosphorylated Thr202 (Syt1) and Thr199 (Syt2) are essential tools for studying the regulatory mechanisms of synaptic transmission under physiological or pathological conditions. They enable researchers to detect dynamic phosphorylation changes in response to neuronal activity, pharmacological interventions, or disease states (e.g., neurodegenerative disorders). Such antibodies are commonly validated via techniques like immunoblotting, immunofluorescence, or immunohistochemistry using knockout controls or phosphatase-treated samples to confirm specificity. Understanding Syt1/Syt2 phosphorylation patterns provides insights into synaptic dysregulation in conditions like epilepsy, Alzheimer’s disease, or schizophrenia, where synaptic vesicle dynamics are compromised.
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