Rabbit Polyclonal HDAC4(Phospho-Ser632) Antibody

The HDAC4 (Phospho-Ser632) antibody is a specialized tool used to detect histone deacetylase 4 (HDAC4) when phosphorylated at serine residue 632. HDAC4. a member of the class IIa HDAC family, regulates gene expression by removing acetyl groups from histones, leading to chromatin condensation and transcriptional repression. It plays critical roles in cellular processes such as muscle differentiation, neuronal survival, and bone development. Phosphorylation at Ser632 (or Ser631 in some numbering systems) is a key post-translational modification that influences HDAC4 subcellular localization. In unphosphorylated states, HDAC4 resides in the nucleus, where it interacts with transcription factors like MEF2 to repress target genes. Upon phosphorylation—often mediated by calcium/calmodulin-dependent kinases (CaMKs) or other kinases—HDAC4 is exported to the cytoplasm, relieving repression of its target genes. This phosphorylation event is crucial in signaling pathways responsive to cellular stress, metabolic changes, or developmental cues. The HDAC4 (Phospho-Ser632) antibody is widely used in research to study HDAC4 dynamics in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. Its specificity enables the detection of phosphorylation-dependent regulatory mechanisms in HDAC4 activity, offering insights into therapeutic targeting of HDAC4-related pathways. Validation typically includes Western blotting and immunofluorescence to confirm reactivity with phosphorylated HDAC4 in relevant biological samples.