| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HD2 |
| Entrez GeneID | 3066; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC2(Phospho-Ser394)抗体的示例参考文献(注：以下为虚构文献，仅供格式参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Phosphorylation of HDAC2 at Ser394 Modulates Its Interaction with p53 in Alzheimer's Disease Models*
**作者**：Smith A, et al.
**摘要**：研究利用HDAC2(Phospho-Ser394)抗体，发现阿尔茨海默病模型中该位点的磷酸化增强，导致HDAC2与p53的相互作用减弱，进而影响神经元凋亡通路。
2. **文献名称**：*CK1-Dependent Phosphorylation of HDAC2 Ser394 Regulates Chromatin Remodeling in Cancer Cells*
**作者**：Lee B, et al.
**摘要**：通过特异性抗体检测，证实CK1激酶介导的Ser394磷酸化抑制HDAC2的脱乙酰酶活性，促进染色质松弛并增强癌症细胞的化疗耐药性。
3. **文献名称**：*HDAC2 Phosphorylation at Ser394 Impairs DNA Repair by Modulating HDAC2-NuRD Complex Formation*
**作者**：Zhang X, et al.
**摘要**：研究发现DNA损伤后，HDAC2的Ser394磷酸化水平升高(经Western blot验证)，阻碍其与NuRD复合体结合，从而延缓DNA修复过程。
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**建议**：实际研究中，可通过以下关键词在PubMed/Google Scholar检索：
`"HDAC2 Ser394 phosphorylation"`、`"HDAC2 Phospho-Ser394 antibody"`、`"HDAC2 post-translational modification"`，筛选涉及表观遗传调控、疾病模型或激酶信号通路的文献。
**Background of HDAC2 (Phospho-Ser394) Antibody**
Histone deacetylase 2 (HDAC2) is a member of the class I HDAC family, which regulates gene expression by removing acetyl groups from histones, promoting chromatin condensation and transcriptional repression. HDAC2 plays critical roles in cell cycle progression, apoptosis, and differentiation, with dysregulation linked to cancer, neurodegenerative disorders, and metabolic diseases. Post-translational modifications, including phosphorylation, modulate its activity, stability, and interactions.
The phosphorylation of HDAC2 at Ser394 (Ser394 in humans, equivalent to Ser393 in mice) is implicated in cellular responses to DNA damage and oxidative stress. This modification enhances HDAC2’s enzymatic activity and nuclear retention, influencing its ability to repress pro-survival or pro-apoptotic genes. Studies suggest that phosphorylation at this site may regulate HDAC2’s role in chromatin remodeling and its interaction with partner proteins during stress signaling.
The HDAC2 (Phospho-Ser394) antibody is a specialized tool designed to detect endogenous HDAC2 protein when phosphorylated at Ser394. It is typically generated using synthetic phosphopeptides corresponding to the phosphorylation site as immunogens. Validated applications include Western blotting, immunohistochemistry, and immunoprecipitation. Specificity is confirmed using phosphatase-treated samples or HDAC2 knockout controls.
This antibody is widely used to study HDAC2 regulation in DNA damage response pathways, epigenetic mechanisms in disease models, and the impact of kinase/phosphatase signaling on HDAC2 function. Researchers employ it to explore therapeutic targets, particularly in oncology and neurology, where HDAC2 phosphorylation may influence drug resistance or disease progression. Proper sample preparation (e.g., avoiding phosphatase inhibitors) and controls are critical for accurate detection.
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