关键信息
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基因名
crtX
- 应用
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别名
crtX；ugt101；Zeaxanthin glucosyltransferase
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P21686
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表达区间
1-431aa
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氨基酸序列
MSHFAAIAPPFYSHVRALQNLAQELVARGHRVTFIQQYDIKHLIDSETIGFHSVGTDSHPPGALTRVLHLAAHPLGPSMLKLINEMARTTDMLCRELPQAFNDLAVDGVIVDQMEPAGALVAEALGLPFISVACALPLNREPDMPLAVMPFEYGTSDAARERYAASEKIYDWLMRRHDRVIAEHSHRMGLAPRQKLHQCFSPLAQISQLVPELDFPRKALPACFHAVGPLRETHAPSTSSSRYFTSSEKPRIFASLGTLQGHRYGLFKTIVKACEEIDGQLLLAHCGRLTDSQCEELARSRHTQVVDFADQSAALSQAQLAITHGGMNTVLDAINYRTPLLALPLAFDQPGVASRIVYHGIGKRASRFTTSHALARQMRSLLTNVDFQQRMAKIQTALRLAGGTMAAADIIEQVMCTGQPVLSGSGYATAL
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分子量
54.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
crtX, a gene encoding a recombinant protein, has gained attention in the context of microbial physiology and biotechnology due to its role in the carotenoid biosynthetic pathway. Carotenoids are important pigments that not only contribute to the coloration of various organisms but also play a crucial role in photoprotection and antioxidant activity. The exploration of crtX is pertinent as it is involved in the enzymatic conversion of precursors into carotenoid compounds, which are vital for the survival and adaptability of certain microorganisms under stress conditions. Research on crtX has implications for agricultural practices, as carotenoids have been shown to enhance the nutritional value of crops and improve their resistance to environmental stresses. Furthermore, understanding crtX function can lead to advances in metabolic engineering, allowing for the optimization of carotenoid production in microbial systems for commercial applications. By elucidating the mechanisms of crtX, scientists aim to harness its potential for developing biofortified crops and producing valuable carotenoid compounds in a sustainable manner, contributing to food security and health benefits. The study of crtX recombinant protein thus sits at the intersection of microbiology, genetics, and agricultural science, opening avenues for innovative biotechnological solutions.
