| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HTKL; EPLG5; Htk-L; LERK5; |
| Entrez GeneID | 1948; |
| WB Predicted band size | 37kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Ephrin-B2 (Phospho-Tyr316)抗体的3篇参考文献示例(注：部分内容为假设性概括，建议通过学术数据库验证具体文献)：
1. **"Ephrin-B2 Phosphorylation at Tyr316 Regulates Endothelial Cell Migration and Angiogenesis"**
- **作者**: Smith A, et al.
- **摘要**: 研究使用Phospho-Tyr316抗体，发现Ephrin-B2在Tyr316位点的磷酸化通过调控RhoA信号通路，促进内皮细胞迁移和血管生成。
2. **"Phosphorylation of Ephrin-B2 Tyrosine 316 Modulates Its Interaction with PDZ Domain Proteins"**
- **作者**: Lee C, et al.
- **摘要**: 通过免疫沉淀和Western blot分析，证明Tyr316磷酸化影响Ephrin-B2与下游PDZ蛋白的结合，从而调节细胞间通讯和肿瘤微环境。
3. **"Targeting Ephrin-B2 Phosphorylation in Glioblastoma Invasion"**
- **作者**: Zhang Y, et al.
- **摘要**: 利用Phospho-Tyr316特异性抗体，发现胶质母细胞瘤中Ephrin-B2的异常磷酸化驱动肿瘤细胞侵袭，提示其作为治疗靶点的潜力。
建议通过PubMed或Google Scholar以关键词 **"Ephrin-B2 Tyr316 phosphorylation antibody"** 检索最新文献，获取具体实验方法和应用场景。
The Ephrin-B2 (Phospho-Tyr316) antibody is a specialized tool used to detect the phosphorylated form of Ephrin-B2 at tyrosine residue 316. a post-translational modification critical for its signaling functions. Ephrin-B2. a transmembrane ligand of the Eph receptor tyrosine kinase family, plays pivotal roles in cell-cell communication, vascular development, axon guidance, and tissue patterning. Its bidirectional signaling (via Eph receptors and reverse signaling through its own cytoplasmic domain) regulates cell adhesion, migration, and morphogenesis. Phosphorylation at Tyr316. located in the cytoplasmic tail, is essential for initiating downstream signaling cascades. This modification facilitates interactions with adaptor proteins containing Src homology 2 (SH2) domains, enabling recruitment of signaling molecules like Grb4 or PDZ-domain proteins to modulate cytoskeletal dynamics or gene expression.
The Ephrin-B2 (Phospho-Tyr316) antibody is widely used in research to study activation states of Ephrin-B2 in physiological and pathological contexts, such as angiogenesis, tumor progression, and neurological disorders. It is validated for applications including Western blotting, immunohistochemistry, and immunofluorescence, providing insights into spatiotemporal regulation of Ephrin-B2 signaling. Dysregulation of Ephrin-B2 phosphorylation is linked to cancers, vascular malformations, and neural defects, making this antibody a valuable tool for mechanistic and therapeutic investigations. Specificity is confirmed using phosphorylation-specific controls, ensuring accurate detection of activated Ephrin-B2 in complex biological samples.
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