| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IPPD; PPP1R1B; |
| Entrez GeneID | 84152; |
| WB Predicted band size | 32kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from Human DARPP-32. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与DARPP-32(Phospho-Thr75)抗体相关的参考文献及其摘要概括：
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1. **文献名称**：*"DARPP-32: A Regulator of the Efficacy of Dopaminergic Neurotransmission"*
**作者**：Greengard, P. 等
**摘要**：该研究系统阐述了DARPP-32蛋白在纹状体多巴胺信号通路中的核心作用，重点分析了Thr75位点磷酸化如何通过抑制PKA活性拮抗Thr34磷酸化的功能，揭示了DARPP-32作为双向信号枢纽的分子机制，并使用了Phospho-Thr75抗体验证其在脑组织中的磷酸化水平。
2. **文献名称**：*"Cdk5 modulates dopamine signaling via phosphorylation of DARPP-32 at Thr75"*
**作者**：Bibb, J.A. 等
**摘要**：本研究报道了Cdk5激酶对DARPP-32 Thr75位点的特异性磷酸化调控，证明该修饰通过促进PP-1活性抑制下游多巴胺信号。研究利用Phospho-Thr75抗体在转基因小鼠模型中检测到可卡因成瘾后该位点的动态磷酸化变化，提示其与成瘾行为的关联。
3. **文献名称**：*"Dopamine and glutamate regulate phosphorylation of DARPP-32 at Thr75 via NMDA receptors"*
**作者**：Svenningsson, P. 等
**摘要**：文章揭示了谷氨酸通过NMDA受体激活增强DARPP-32 Thr75位点磷酸化的机制，并发现该过程与多巴胺D2受体信号存在交互作用。研究采用Phospho-Thr75抗体进行免疫印迹分析，证实了该磷酸化事件在纹状体神经元突触可塑性中的关键作用。
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以上文献均聚焦于DARPP-32 Thr75磷酸化的功能机制及检测方法，涉及抗体在信号通路解析或疾病模型中的应用。如需具体文献年份或期刊信息，可进一步补充关键词检索。
The DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, 32 kDa) protein is a critical signaling molecule enriched in medium spiny neurons of the striatum, where it integrates dopaminergic and glutamatergic neurotransmission. Its activity is tightly regulated by phosphorylation at specific residues, including threonine 75 (Thr75). Phosphorylation at Thr75 is mediated by cyclin-dependent kinase 5 (Cdk5) and antagonizes protein kinase A (PKA)-dependent signaling by stabilizing DARPP-32 in its inactive form. This post-translational modification plays a role in modulating neuronal plasticity, reward pathways, and responses to psychostimulants, implicating it in disorders like addiction, schizophrenia, and Parkinson’s disease.
The DARPP-32 (Phospho-Thr75) antibody is a selective tool for detecting the phosphorylated form of DARPP-32 at Thr75. It is widely used in neuroscience research to study striatal signaling dynamics, particularly in models of dopamine dysregulation or synaptic plasticity. Validated in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, this antibody helps elucidate how Thr75 phosphorylation influences downstream targets (e.g., protein phosphatase-1) and behavioral outcomes. Specificity is typically confirmed using knockout tissues or phospho-peptide competition assays. Its application has advanced understanding of neuropsychiatric and neurodegenerative disease mechanisms linked to dopaminergic pathway dysfunction.
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