| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1 |
| Entrez GeneID | 7534; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于14-3-3ζ (Phospho-Ser58)抗体的参考文献示例(内容为虚构，仅供参考格式)：
1. **"Phosphorylation of 14-3-3ζ at Ser58 modulates apoptosis in cancer cells"**
*作者：Zhang L, et al.*
**摘要**：研究揭示了14-3-3ζ的Ser58磷酸化通过调控Bcl-2家族蛋白的相互作用促进肿瘤细胞凋亡，并利用Phospho-Ser58抗体验证了该位点在临床样本中的表达与预后相关性。
2. **"Development of a specific antibody for 14-3-3ζ phosphorylated at Ser58 and its application in neurodegenerative models"**
*作者：Smith J, et al.*
**摘要**：报道了一种高特异性的Phospho-Ser58抗体，通过肽段免疫和磷酸化位点突变验证其选择性，并应用于阿尔茨海默病模型中14-3-3ζ磷酸化水平的检测。
3. **"AKT-mediated phosphorylation of 14-3-3ζ at Ser58 enhances its binding to Raf-1"**
*作者：Wang Y, et al.*
**摘要**：证明AKT激酶磷酸化14-3-3ζ的Ser58位点，通过Phospho-Ser58抗体和免疫共沉淀实验揭示该修饰增强了14-3-3ζ与Raf-1的结合，进而调控MAPK信号通路活性。
4. **"Phospho-Ser58 antibody reveals dynamic 14-3-3ζ modification during cell cycle progression"**
*作者：Chen R, et al.*
**摘要**：利用Phospho-Ser58抗体的免疫荧光技术，发现14-3-3ζ在G2/M期特异性磷酸化，提示其可能参与细胞周期检查点调控。
(注：以上文献为模拟生成，实际引用需查询PubMed等数据库获取真实信息。)
The 14-3-3ζ (Phospho-Ser58) antibody is a specialized tool used to detect the phosphorylated form of the 14-3-3ζ protein at serine residue 58 (Ser58). The 14-3-3 protein family comprises conserved regulatory molecules involved in diverse cellular processes, including signal transduction, apoptosis, cell cycle control, and stress responses. These proteins typically function as homo- or heterodimers, binding to phosphorylated serine/threonine motifs on target proteins to modulate their activity, localization, or stability. The ζ isoform (14-3-3ζ) is widely expressed and implicated in cancer progression, neurodegenerative diseases, and metabolic regulation.
Phosphorylation of Ser58 in 14-3-3ζ is a post-translational modification that may regulate its dimerization, substrate-binding affinity, or intracellular trafficking. This modification is thought to alter the protein's conformation, potentially affecting interactions with client proteins or its own stability. The 14-3-3ζ (Phospho-Ser58) antibody is generated using synthetic peptides corresponding to the phosphorylated Ser58 epitope, enabling specific recognition of this modified form. It is commonly employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study phosphorylation-dependent 14-3-3ζ functions in cellular signaling pathways. Researchers utilize this antibody to explore mechanisms underlying diseases where 14-3-3ζ dysregulation or aberrant phosphorylation is implicated, such as cancer metastasis or neurodegenerative disorders. Specificity validation, including knockout controls and phosphatase treatment, is essential to confirm accurate detection.
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