| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; MAPKK 4 |
| Entrez GeneID | 6416; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 80 (T-H-S(p)-I-E) derived from Human SEK1/MKK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SEK1/MKK4(Phospho-Ser80)抗体的参考文献示例(内容基于假设性研究，供参考)：
1. **文献名称**：*Phosphorylation of MKK4 at Serine 80 Regulates JNK Activation in Response to Cellular Stress*
**作者**：Smith A, et al.
**摘要**：本研究揭示了MKK4在Ser80位点的磷酸化对JNK信号通路的调控作用，利用Phospho-Ser80特异性抗体验证了氧化应激下该位点的修饰与细胞凋亡的相关性。
2. **文献名称**：*SEK1/MKK4 Ser80 Phosphorylation as a Biomarker in Breast Cancer Progression*
**作者**：Chen L, et al.
**摘要**：通过免疫组化分析，发现Phospho-Ser80抗体标记的MKK4在乳腺癌组织中高表达，提示其可能作为肿瘤侵袭性的潜在标志物。
3. **文献名称**：*Structural Basis of MKK4 Activation: Role of Ser80 Phosphorylation in Kinase Conformation*
**作者**：Yamamoto K, et al.
**摘要**：结合X射线晶体学与Western blot(使用Phospho-Ser80抗体)，阐明Ser80磷酸化诱导MKK4构象变化，从而增强其下游底物结合能力。
4. **文献名称**：*A Functional Screen Identifies MKK4 Ser80 as a Critical Site for Apoptotic Signaling*
**作者**：Wang X, et al.
**摘要**：通过CRISPR/Cas9和Phospho-Ser80抗体检测，证明Ser80磷酸化缺失显著抑制紫外线诱导的细胞凋亡，提示其在DNA损伤应答中的必要性。
**注意**：以上文献为示例，实际引用需通过PubMed、Google Scholar等平台以关键词“MKK4 Ser80 phosphorylation antibody”检索确认。部分商业抗体厂商(如Cell Signaling Technology)的技术文档也可能提供相关应用案例。
The SEK1/MKK4 (Phospho-Ser80) antibody is designed to detect the activated form of the dual-specificity kinase MKK4 (MAP Kinase Kinase 4), also known as SEK1 (SAPK/ERK Kinase 1), when phosphorylated at serine 80. MKK4 is a critical component of the stress-activated protein kinase (SAPK) signaling pathway, primarily functioning upstream of c-Jun N-terminal kinase (JNK) and p38 MAPK. It plays a pivotal role in mediating cellular responses to stress stimuli, cytokines, and growth factors, regulating processes like apoptosis, differentiation, and inflammation.
Phosphorylation at Ser80 is associated with the activation or regulatory modulation of MKK4. though its exact functional implications remain under investigation. This antibody enables researchers to specifically identify and quantify the phosphorylated (active) form of MKK4 in experimental models, facilitating studies on stress signaling dynamics, kinase cascades, and disease mechanisms. It is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore MKK4's role in cancer, neurodegenerative disorders, and immune responses. Dysregulation of MKK4 has been implicated in tumor progression and resistance to therapy, making this antibody a valuable tool for both basic research and translational studies aiming to uncover therapeutic targets.
×
