关键信息
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基因名
gGT1
- 应用
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别名
gGT1；GGT；Glutathione hydrolase 1 proenzyme
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19440
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表达区间
28-569aa
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氨基酸序列
MASMTGGQQMGRGHHHHHHGNLYFQGGESASKEPDNHVYTRAAVAADAKQ CSKIGRDALRDGGSAVDAAIAALLCVGLMNAHSMGIGGGLFLTIYNSTTR KAEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRGYELAHQRHG RLPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDR KVLREGERLTLPQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVT AEDLNNYRAELIEHPLNISLGDVVLYMPSAPLSGPVLALILNILKGYNFS RESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEF FAAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATST INLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQP LSSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLWFGYDVKRAV EEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVR TAGGWAAASDSRKGGEPAGY
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分子量
62 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GGT1, or gamma-glutamyltransferase 1, is an enzyme that plays a crucial role in glutathione metabolism and the detoxification of reactive oxygen species. It is predominantly expressed in various human tissues, particularly in the liver and kidney, where it is involved in the maintenance of cellular redox status and the metabolism of extracellular glutathione. Elevated levels of GGT1 have been associated with several pathological conditions, including liver diseases, metabolic syndrome, and certain types of cancer, making it a potential biomarker for disease diagnosis and progression. Research on recombinant GGT1 protein has gained traction due to its significance in therapeutic applications and its role in understanding the biochemical pathways involving glutathione. By producing and characterizing GGT1 in a recombinant system, researchers aim to elucidate its functional properties, enzyme kinetics, and potential inhibitors, thus providing insights into its biological role and developing strategies for drug design. Furthermore, recombinant GGT1 can serve as a valuable tool for studying its involvement in disease mechanisms, screening for modulators of its activity, and exploring its therapeutic potential in oxidative stress-related disorders. Overall, the study of GGT1 and its recombinant protein presents a promising avenue for advancing our knowledge in biochemistry and translational medicine.
