| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PLC-IV; PLC-gamma2; Phospholipase C-gamma-2 |
| Entrez GeneID | 5336; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLCg2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLCγ2 (Phospho-Tyr753)抗体的参考文献示例(注：文献为假设性概括，实际文献需通过数据库验证)：
1. **文献名称**: *"B Cell Receptor-Induced Phosphorylation of Phospholipase Cγ2 at Tyr753 Regulates Calcium Signaling"*
**作者**: Kurosaki, T. et al.
**摘要**: 本研究揭示了B细胞受体(BCR)激活后，PLCγ2在Tyr753位点的磷酸化对下游钙离子信号的关键作用，通过特异性抗体证实了该磷酸化事件与Syk激酶的依赖性关系。
2. **文献名称**: *"Constitutive PLCγ2 Activation in Chronic Lymphocytic Leukemia via Tyr753 Phosphorylation"*
**作者**: Smith, J. et al.
**摘要**: 研究发现慢性淋巴细胞白血病(CLL)患者中PLCγ2的Tyr753位点异常磷酸化，导致组成性激活。使用Phospho-Tyr753抗体检测到该现象与疾病进展相关。
3. **文献名称**: *"Phosphospecific Antibody Characterization for Detecting PLCγ2 Activation in Immune Cells"*
**作者**: Johnson, R. et al.
**摘要**: 本文描述了Phospho-Tyr753抗体的开发与验证，证明其在流式细胞术和Western blot中特异性识别活化的PLCγ2.为免疫信号研究提供工具。
4. **文献名称**: *"Role of PLCγ2 Tyr753 Phosphorylation in Neutrophil Chemotaxis"*
**作者**: Lee, H. et al.
**摘要**: 通过Phospho-Tyr753抗体，研究发现中性粒细胞趋化过程中PLCγ2的Tyr753磷酸化调控细胞极性及迁移，揭示其非经典信号功能。
**建议**：实际文献需通过PubMed或Google Scholar以关键词“PLCγ2 Tyr753 phosphorylation antibody”检索，并筛选涉及抗体应用或机制的研究。
The phospholipase C gamma 2 (PLCγ2) antibody targeting the phosphorylated tyrosine residue at position 753 (Tyr753) is a critical tool for studying intracellular signaling pathways, particularly in immune cells. PLCγ2. a key enzyme in the phosphoinositide signaling cascade, is activated upon phosphorylation at specific tyrosine residues, including Tyr753. This phosphorylation event, mediated by tyrosine kinases such as Syk or Btk in response to antigen receptor engagement (e.g., B-cell receptor), triggers PLCγ2's enzymatic activity. Activated PLCγ2 hydrolyzes phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers (IP3 and DAG), driving downstream processes like calcium mobilization and protein kinase C (PKC) activation, which regulate immune cell proliferation, differentiation, and function.
The PLCγ2 (Phospho-Tyr753) antibody specifically detects this post-translational modification, enabling researchers to monitor PLCγ2 activation status in signaling studies. It is widely used in immunology and oncology research to investigate B-cell signaling, immune regulation, and pathological mechanisms in diseases like immunodeficiencies, autoimmune disorders, and lymphomas. Validated in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, this antibody helps elucidate how dysregulated PLCγ2 activity contributes to disease progression or therapeutic resistance. Its specificity for the phosphorylated form ensures accurate detection of the enzyme's active state, making it indispensable for dissecting tyrosine kinase-dependent signaling networks.
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