关键信息
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基因名
TRMT10C
- 应用
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别名
TRMT10C；MRPP1；RG9MTD1；tRNA methyltransferase 10 homolog C
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7L0Y3
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表达区间
40-403aa
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氨基酸序列
MSSKIPAVTYPKNESTPPSEELELDKWKTTMKSSVQEECVSTISSSKDEDPLAATREFIEMWRLLGREVPEHITEEELKTLMECVSNTAKKKYLKYLYTKEKVKKARQIKKEMKAAAREEAKNIKLLETTEEDKQKNFLFLRLWDRNMDIAMGWKGAQAMQFGQPLVFDMAYENYMKRKELQNTVSQLLESEGWNRRNVDPFHIYFCNLKIDGALHRELVKRYQEKWDKLLLTSTEKSHVDLFPKDSIIYLTADSPNVMTTFRHDKVYVIGSFVDKSMQPGTSLAKAKRLNLATECLPLDKYLQWEIGNKNLTLDQMIRILLCLKNNGNWQEALQFVPKRKHTGFLEISQHSQEFINRLKKAKT
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分子量
46.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TRMT10C, or tRNA methyltransferase 10C, is a crucial enzyme involved in the post-transcriptional modification of tRNA, specifically in the methylation of adenosine residues, which enhances the stability and functionality of tRNA molecules during protein synthesis. Research on TRMT10C has gained prominence due to its implications in various cellular processes and its association with several human diseases. Mutations in the TRMT10C gene have been linked to developmental disorders and neurological conditions, highlighting its importance in maintaining cellular homeostasis and proper organismal development. Understanding the biochemical mechanisms through which TRMT10C operates can provide valuable insights into the fundamental roles of tRNA modifications in translational control and gene expression regulation. Moreover, the study of TRMT10C has potential therapeutic implications, as targeting tRNA methylation pathways may offer novel strategies for treating diseases related to tRNA dysfunction. Consequently, recombinant expression and characterization of TRMT10C can pave the way for further investigations into its function, regulation, and potential as a drug target, thus establishing its significance in both basic and applied biomedical research.
