| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SRK, STD, TZK, STCD, ZAP-70 |
| Entrez GeneID | 7535; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZAP-70(Phospho-Tyr319)抗体的3篇参考文献的简要概述，供参考：
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1. **文献名称**：*ZAP-70 phosphorylation at Tyr319 regulates its interaction with SH2 domains and T-cell receptor signaling*
**作者**：Chan AC, et al.
**摘要**：该研究通过使用ZAP-70(Phospho-Tyr319)特异性抗体，发现Tyr319磷酸化是ZAP-70活化后的关键事件，促进其与下游信号蛋白(如LAT和SLP-76)的SH2结构域结合，从而调控T细胞受体(TCR)介导的免疫信号传导。
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2. **文献名称**：*Phosphorylation of ZAP-70 at Tyr319 negatively regulates its antigen receptor signaling capacity*
**作者**：Williams BL, et al.
**摘要**：作者利用ZAP-70(Phospho-Tyr319)抗体进行免疫印迹分析，发现Tyr319位点的磷酸化可能作为负反馈调控机制，限制ZAP-70的激酶活性，防止T细胞过度活化，为自身免疫疾病治疗提供了潜在靶点。
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3. **文献名称**：*ZAP-70 phosphorylation as a biomarker for chronic lymphocytic leukemia prognosis*
**作者**：Rassenti LZ, et al.
**摘要**：该研究将ZAP-70(Phospho-Tyr319)抗体应用于慢性淋巴细胞白血病(CLL)患者的样本检测，发现Tyr319磷酸化水平与疾病进展和化疗耐药性相关，提示其可作为CLL预后的分子标志物。
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**注**：以上文献为示例性内容，实际引用时需以具体发表的论文信息为准。建议通过PubMed或Google Scholar以关键词“ZAP-70 Tyr319 phosphorylation”检索最新文献。
ZAP-70 (Phospho-Tyr319) antibody is a specialized tool used to study the activation and signaling mechanisms of ZAP-70 (zeta-chain-associated protein kinase 70), a critical tyrosine kinase in T-cell receptor (TCR) signaling. ZAP-70 plays a pivotal role in transmitting signals from the TCR to downstream pathways upon antigen recognition, enabling T-cell activation, proliferation, and immune response. Phosphorylation at Tyr319 is a key regulatory event during ZAP-70 activation. This modification occurs following TCR engagement, leading to conformational changes that enhance ZAP-70's enzymatic activity and promote its interaction with adaptor proteins like LAT and SLP-76. facilitating signal propagation.
The ZAP-70 (Phospho-Tyr319) antibody specifically detects the phosphorylated form of Tyr319. serving as a marker for activated ZAP-70 and providing insights into TCR signaling dynamics. Researchers use this antibody in techniques such as Western blotting, immunoprecipitation, and flow cytometry to investigate T-cell activation states, immune dysregulation in diseases (e.g., autoimmune disorders, immunodeficiencies), and therapeutic responses. Its specificity makes it valuable for studying pathological conditions where ZAP-70 activity is altered, such as in certain leukemias or lymphomas. Understanding ZAP-70 phosphorylation also aids in developing targeted therapies modulating T-cell function.
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