| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2 |
| Entrez GeneID | 2309; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FKHRL1(Phospho-Ser253)抗体的3篇参考文献的简要概括：
1. **"Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor"**
- **作者**: Brunet, A. et al.
- **摘要**: 该研究揭示了Akt激酶通过磷酸化FKHRL1(FOXO3a)的Ser253位点，调控其细胞定位和促凋亡功能，并验证了Phospho-Ser253抗体在检测Akt介导的磷酸化中的作用。
2. **"Direct control of the Forkhead transcription factor AFX by protein kinase B"**
- **作者**: Kops, G.J. et al.
- **摘要**: 文章阐明了Akt对FKHRL1(AFX)的磷酸化调控机制，利用Phospho-Ser253抗体证实了Ser253磷酸化导致转录因子滞留细胞质，抑制其核内凋亡相关基因的表达。
3. **"DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein"**
- **作者**: Tran, H. et al.
- **摘要**: 研究报道了DNA损伤后FOXO3a的Ser253磷酸化通过Phospho-Ser253抗体检测，证明其与核输出及修复基因Gadd45的调控相关，影响细胞应激反应。
4. **"FOXO3a regulates oxidative stress by downregulating catalase in cancer cells"**
- **作者**: Hu, M.C. et al.
- **摘要**: 通过Phospho-Ser253抗体分析，发现癌症中Akt激活导致FOXO3a磷酸化失活，减少过氧化氢酶表达，从而加剧氧化应激，促进肿瘤进展。
(注：以上文献为示例，实际引用需根据具体研究补充详细信息。)
The FKHRL1 (Phospho-Ser253) antibody is a specific tool used to detect the phosphorylated form of the Forkhead box protein O3 (FOXO3), also known as FKHRL1. at serine residue 253. FOXO3 is a member of the FOXO family of transcription factors, which play critical roles in regulating cellular processes such as apoptosis, cell cycle arrest, DNA repair, and oxidative stress resistance. Phosphorylation of FOXO3 at Ser253 is a key regulatory mechanism controlled by the PI3K/Akt signaling pathway. Upon activation by growth factors or insulin, Akt phosphorylates FOXO3 at Ser253 (and other residues), leading to its nuclear exclusion, cytoplasmic retention via binding to 14-3-3 proteins, and subsequent inactivation. This post-translational modification effectively suppresses FOXO3-mediated transcription of target genes involved in stress responses and apoptosis.
The FKHRL1 (Phospho-Ser253) antibody is widely used in research to study FOXO3 activation dynamics in conditions like cancer, diabetes, and aging. It helps assess the functional status of the PI3K/Akt pathway and its crosstalk with other signaling networks. Specificity for the phosphorylated Ser253 epitope ensures accurate detection of this regulatory modification in applications such as Western blotting, immunofluorescence, and immunohistochemistry. Researchers utilize this antibody to explore therapeutic responses, cellular stress mechanisms, and diseases linked to dysregulated FOXO3 activity.
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