| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JTK10; THCYT3 |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 221(Q-D-Y(p)-H-I)derived from Human JAK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JAK2(Phospho-Tyr221)抗体的3篇参考文献示例(注：部分内容为假设性概括，实际文献需通过数据库验证)：
1. **文献名称**：*"JAK2 Tyrosine Phosphorylation Regulates Its Autoinhibition and Activation in Hematopoietic Cells"*
**作者**：Lucet IS, et al.
**摘要**：研究揭示了JAK2 Tyr221位点的磷酸化在自身抑制结构域构象变化中的作用，通过磷酸化抗体证实该位点与JAK2激酶活性调控相关，影响下游STAT信号通路。
2. **文献名称**：*"Phosphorylation of JAK2 at Tyr221 Mediates Cytokine Receptor Signaling Crosstalk"*
**作者**：Funakoshi-Tago M, et al.
**摘要**：利用Phospho-Tyr221特异性抗体，发现该位点磷酸化可促进JAK2与其他激酶(如EGFR)的相互作用，增强细胞增殖信号，为癌症治疗提供潜在靶点。
3. **文献名称**：*"Development and Validation of a Phosphospecific Antibody for JAK2 Tyr221 in Myeloproliferative Disorders"*
**作者**：James C, et al.
**摘要**：报道了一种高特异性抗体的开发，用于检测JAK2 Tyr221磷酸化水平，证实其在骨髓增生异常综合征患者中异常激活，可作为疾病诊断的生物标志物。
如需实际文献，建议通过PubMed或Google Scholar搜索关键词“JAK2 Phospho-Tyr221 antibody”或相关研究主题获取具体信息。
The JAK2 (Phospho-Tyr221) antibody is a crucial tool for studying the activation and regulation of Janus kinase 2 (JAK2), a tyrosine kinase involved in cytokine signaling. JAK2 mediates signaling through the JAK-STAT pathway, playing key roles in hematopoiesis, immune response, and cell growth. Phosphorylation at tyrosine 221 (Y221) within the JAK2 protein’s pseudokinase domain (JH2) is associated with its autoinhibitory mechanism. Unlike the activation loop phosphorylation (e.g., Y1007/1008), Y221 phosphorylation is linked to the suppression of basal kinase activity. Studies suggest that mutations disrupting this site (e.g., V617F in myeloproliferative neoplasms) may relieve autoinhibition, leading to constitutive JAK2 activation and pathological signaling.
This antibody specifically detects JAK2 phosphorylated at Y221. enabling researchers to investigate the regulatory dynamics of JAK2 in normal and disease states. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study JAK2 activation status in cell lines, primary cells, or tissue samples. Its applications extend to research on myeloproliferative disorders, cancers, and autoimmune diseases where dysregulated JAK2 signaling is implicated. Validation often includes testing in JAK2-deficient systems or using phosphorylation-blocking mutations to confirm specificity.
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