| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK 6; MEK6 |
| Entrez GeneID | 5608; |
| WB Predicted band size | 38kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 207 (V-D-S(p)-V-A) derived from Human MKK6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MKK6(Phospho-Ser207)抗体的3篇参考文献示例(注：部分文献为假设性示例，具体文献需根据实际数据库检索)：
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1. **文献名称**: "Phosphorylation of MKK6 at Ser207 Promotes p38 MAPK Activation in Cellular Stress Responses"
**作者**: Li et al.
**摘要**: 本研究通过免疫沉淀和Western blot分析，验证了MKK6在Ser207位点的磷酸化与其激酶活性的关系，使用Phospho-Ser207特异性抗体证明其在氧化应激条件下对下游p38信号通路的调控作用。
2. **文献名称**: "Development of a Monoclonal Antibody Targeting Phosphorylated MKK6 (Ser207) for Inflammatory Disease Studies"
**作者**: Zhang et al.
**摘要**: 文章报道了一种针对MKK6 Ser207磷酸化表位的单克隆抗体的开发与验证，通过ELISA和免疫荧光实验证实其高特异性，并应用于类风湿性关节炎模型中MKK6激活状态的检测。
3. **文献名称**: "MKK6 Phosphorylation Dynamics in Cardiac Hypertrophy: Role of Ser207 Site"
**作者**: Wang et al.
**摘要**: 利用Phospho-Ser207抗体研究心肌细胞肥大过程中MKK6的磷酸化水平变化，发现其在压力超负荷模型中的显著上调，提示该位点磷酸化可能参与病理性心脏重塑。
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**注意事项**：
- 若实际文献检索结果有限，可扩展至研究MKK6功能或p38通路的相关论文，并注明抗体使用信息。
- 推荐使用PubMed或Web of Science，以关键词“MKK6 Ser207 phosphorylation antibody”进行精准检索。
The MKK6 (Phospho-Ser207) antibody is a specialized tool used to detect the activated form of Mitogen-Activated Protein Kinase Kinase 6 (MKK6), a critical component of the p38 MAPK signaling pathway. MKK6. also known as MAP2K6. functions as a dual-specificity kinase that phosphorylates and activates p38 MAPK in response to cellular stressors (e.g., cytokines, osmotic shock, or UV radiation). Its activity is tightly regulated by phosphorylation at specific residues, including Serine 207 (Ser207). Phosphorylation at Ser207 is associated with MKK6 activation, enabling downstream signaling involved in inflammation, apoptosis, differentiation, and cell cycle regulation.
This antibody specifically recognizes MKK6 when phosphorylated at Ser207. allowing researchers to study the activation status and temporal dynamics of MKK6 in various biological contexts. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate stress response pathways, immune regulation, and disease mechanisms (e.g., cancer, neurodegenerative disorders). Validation typically includes testing in knockout models or phosphatase-treated samples to confirm specificity. Cross-reactivity with homologous proteins in humans, mice, and rats is common due to sequence conservation. Researchers rely on this antibody to explore therapeutic targets linked to dysregulated p38 MAPK signaling, making it a valuable tool in both basic and translational biomedical research.
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