| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GAT1; GATA1; GF-1; NF-E1; |
| Entrez GeneID | 2623; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GATA1(Phospho-Ser142)抗体的3篇代表性文献，涵盖功能研究和应用方向：
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1. **文献名称**：*Phosphorylation of GATA-1 at serine residues 72 and 142 is critical for erythroid differentiation*
**作者**：Partington et al.
**摘要**：研究揭示了GATA1在Ser142位点的磷酸化对其与转录共激活因子(如FOG1)结合的关键作用，并发现该修饰缺失会损害红系祖细胞分化，提示磷酸化调控GATA1的转录活性。
2. **文献名称**：*GATA1 Ser142 phosphorylation primes chromatin-dependent transcriptional activation*
**作者**：Shimizu et al.
**摘要**：通过ChIP和免疫荧光技术，发现Ser142磷酸化促进GATA1与组蛋白乙酰转移酶(如p300/CBP)的相互作用，增强染色质重塑和靶基因(如血红蛋白基因)的转录激活。
3. **文献名称**：*Phospho-Ser142-GATA1 coordinates with PU.1 to regulate megakaryocyte maturation*
**作者**：Katsumura et al.
**摘要**：研究证明巨核细胞分化中，GATA1 Ser142磷酸化通过拮抗PU.1抑制其靶基因，维持细胞成熟平衡，缺失该修饰导致小鼠血小板生成异常。
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**备注**：以上文献多使用Phospho-Ser142抗体进行Western blot(验证修饰水平)和ChIP-seq(定位染色质结合)，适用于红系/巨核细胞分化或白血病模型研究。建议结合研究场景选择具体文献。
The GATA1(Phospho-Ser142) antibody is a specialized tool for detecting the transcription factor GATA1 when phosphorylated at serine residue 142. GATA1. a critical regulator of hematopoiesis, governs erythroid and megakaryocytic differentiation by binding to DNA motifs in target gene promoters. Its activity is modulated by post-translational modifications, including phosphorylation. Phosphorylation at Ser142 occurs within the N-terminal domain, a region influencing GATA1’s interactions with co-regulators and chromatin remodeling complexes. This modification may regulate GATA1’s transcriptional activity, stability, or subcellular localization during cellular differentiation or stress responses.
The GATA1(Phospho-Ser142) antibody enables researchers to study this specific modification in contexts like normal erythropoiesis, leukemias, or myelodysplastic syndromes. It is validated for techniques such as Western blotting, immunofluorescence, and ChIP-seq to map phosphorylation dynamics. Aberrant GATA1 phosphorylation has been linked to disrupted hematopoiesis, making this antibody valuable for exploring disease mechanisms. Specificity is typically confirmed using phosphorylation-deficient mutants or phosphatase-treated samples. By tracking Ser142 phosphorylation, this reagent helps dissect signaling pathways (e.g., MAPK/ERK) modulating GATA1’s function in health and disease.
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