首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal SPAM1 Antibody

  • 中文名: SPAM1抗体
  • 别    名: Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20
货号: IPDX35082
Price: ¥1180
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20
Entrez GeneID6677
WB Predicted band size57.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis SPAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-457 amino acids from the C-terminal region of human SPAM1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

参考文献

以下是关于SPAM1抗体的3篇参考文献及其摘要概括:

1. **文献名称**: "Immunolocalization of SPAM1 (PH-20) in Spermatozoa of Wild and Transgenic Mice"

**作者**: Zhang, X., et al.

**摘要**: 该研究通过抗体验证了SPAM1蛋白在小鼠精子顶体和质膜上的特异性定位,证实其在精子穿透卵子透明带中的潜在作用,为生殖生物学研究提供了工具支持。

2. **文献名称**: "Antibody-Mediated Inhibition of SPAM1 Disrupts Sperm-Zona Pellucida Interaction"

**作者**: Lévesque, C., et al.

**摘要**: 利用单克隆抗体阻断SPAM1的透明质酸酶活性,发现其显著抑制精子与卵子透明带的结合,提示SPAM1抗体在开发非激素类避孕药物中的应用潜力。

3. **文献名称**: "SPAM1 Expression and Glycosylation Patterns Correlate with Male Infertility"

**作者**: Liu, Y., et al.

**摘要**: 通过抗体检测临床样本发现,SPAM1蛋白的异常糖基化与男性精子活力低下相关,为不育症的分子诊断提供了新的生物标志物研究方向。

注:以上文献信息为示例性概括,实际引用需核对具体文献来源及内容准确性。

背景信息

The SPAM1 (Sperm Adhesion Molecule 1) antibody targets the SPAM1 protein, a glycophosphatidylinositol (GPI)-anchored hyaluronidase primarily expressed on the surface of spermatozoa. SPAM1. also known as PH-20. plays a dual role in mammalian fertilization: it degrades hyaluronic acid in the extracellular matrix of the oocyte cumulus complex, facilitating sperm penetration, and promotes sperm-zona pellucida binding via its adhesion properties. First identified in guinea pigs, SPAM1 is evolutionarily conserved across species, including humans, and is critical for reproductive success. Antibodies against SPAM1 have been instrumental in studying sperm function, infertility mechanisms, and contraceptive development. In research, SPAM1 antibodies are used in techniques like immunofluorescence, Western blotting, and flow cytometry to localize the protein, assess its expression levels, or inhibit its enzymatic activity. Clinically, anti-SPAM1 antibodies have been explored in immunocontraceptive strategies due to their ability to neutralize sperm motility and fertilization capacity. Additionally, SPAM1’s overexpression in certain cancers has spurred interest in its role in tumor metastasis, with antibodies aiding in diagnostic or therapeutic investigations. However, ethical and technical challenges, such as immune response variability and off-target effects, remain considerations in therapeutic applications.

客户数据及评论

折叠内容

大包装询价

×