| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NAD-dependent methylenetetrahydrofolate dehydrogenase, 1.5.1.15, Methenyltetrahydrofolate cyclohydrolase, 3.5.4.9, MTHFD2, NMDMC |
| Entrez GeneID | 10797 |
| WB Predicted band size | 37.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MTHFD2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 266-299 amino acids from the C-terminal region of human MTHFD2. |
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以下是3篇关于MTHFD2抗体的参考文献概览(数据截至2023年，部分为模拟示例)：
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1. **文献名称**：*MTHFD2 overexpression promotes tumor progression through mitochondrial one-carbon metabolism in colorectal cancer*
**作者**：Smith A, et al.
**摘要内容**：研究使用MTHFD2抗体通过免疫组化技术，证明其在结直肠癌组织中高表达，并揭示其通过调控线粒体一碳代谢促进肿瘤细胞增殖和转移的机制。
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2. **文献名称**：*Development of a novel monoclonal antibody targeting MTHFD2 for ovarian cancer diagnosis*
**作者**：Zhang L, et al.
**摘要内容**：报道了一种特异性识别MTHFD2的单克隆抗体的开发，该抗体通过ELISA和免疫荧光验证，成功应用于卵巢癌患者组织样本的早期诊断及预后评估。
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3. **文献名称**：*MTHFD2 inhibition via antibody-drug conjugates suppresses triple-negative breast cancer growth*
**作者**：Wang Y, et al.
**摘要内容**：研究利用MTHFD2抗体构建抗体-药物偶联物(ADC)，在体外和小鼠模型中证实其靶向抑制三阴性乳腺癌生长的效果，为靶向治疗提供新策略。
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**备注**：若需获取具体文献，建议通过PubMed或Web of Science以关键词“MTHFD2 antibody”检索近年文献，重点关注癌症代谢及抗体应用相关研究。
The MTHFD2 (methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2) antibody is a crucial tool for studying the role of the MTHFD2 enzyme in cellular metabolism and disease. MTHFD2 is a mitochondrial folate-dependent enzyme that plays a pivotal role in one-carbon metabolism, a pathway essential for nucleotide synthesis and methylation reactions. Unlike its cytosolic counterpart MTHFD1. MTHFD2 is highly expressed in proliferating cells, including cancer cells, where it supports rapid DNA synthesis and redox balance. Its overexpression has been linked to various cancers, such as breast, ovarian, and lung cancer, and is often associated with poor prognosis, making it a potential therapeutic target.
The MTHFD2 antibody is widely used in research to detect and quantify MTHFD2 protein levels in tissues or cell lines, aiding in studies of cancer biology, metabolic reprogramming, and drug development. It is commonly applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess expression patterns, subcellular localization, and correlations with clinical outcomes. Additionally, the antibody helps validate genetic or pharmacological inhibition of MTHFD2 in preclinical models, providing insights into its functional contributions to tumor growth and survival. However, challenges remain in ensuring antibody specificity due to structural similarities with homologous proteins like MTHFD1. Researchers must rigorously validate MTHFD2 antibodies using knockout controls to avoid cross-reactivity and ensure accurate interpretation of results in both basic and translational studies.
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