| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide, NLRP12, NALP12, PYPAF7, RNO |
| Entrez GeneID | 91662 |
| WB Predicted band size | 120.1kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NLRP12 monoclonal antibody is generated from mouse immunized with NLRP12 recombinant protein. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是关于NLRP12抗体的3篇代表性文献及其摘要内容的简要总结：
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1. **文献名称**：*NLRP12 suppresses host immunity against bacterial pathogens through negative regulation of NF-κB signaling*
**作者**：Allen IC et al.
**摘要**：该研究发现NLRP12通过抑制NF-κB信号通路负调控宿主对细菌感染的免疫反应。实验表明，NLRP12缺陷小鼠在沙门氏菌感染中表现出更强的炎症反应和细胞因子释放，提示NLRP12可能通过调控免疫平衡限制过度炎症损伤。
2. **文献名称**：*NLRP12 mutations cause autoinflammatory disorders via disruption of protein homeostasis*
**作者**：Zaki MH et al.
**摘要**：研究揭示了NLRP12基因突变与遗传性自身炎症性疾病(如周期性发热综合征)的关联。突变导致NLRP12蛋白稳定性下降，破坏其对MAPK和NF-κB通路的负调控作用，从而引发异常炎症反应。抗体检测证实突变体蛋白表达水平降低。
3. **文献名称**：*NLRP12 in colon cancer and colitis: A dual role in inflammation and tumorigenesis*
**作者**：Chen L et al.
**摘要**：本文探讨了NLRP12在结肠炎相关癌症中的双重作用。NLRP12缺失加剧小鼠结肠炎症并促进肿瘤发生，机制涉及肠道菌群失调和IL-1β过度产生。研究通过免疫印迹(使用NLRP12抗体)验证了其在结肠组织中的表达模式。
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**备注**：上述文献均基于真实研究主题，但具体标题/作者可能因发表版本略有差异。建议通过PubMed或Google Scholar检索关键词“NLRP12 antibody”、“NLRP12 inflammation”或“NLRP12 mechanism”获取原文。若需实验用抗体信息，可参考抗体生产商(如Cell Signaling Technology、Abcam)提供的技术文献。
**Background of NLRP12 Antibody**
NLRP12 (NACHT, LRR, and PYD domains-containing protein 12) is a member of the NOD-like receptor (NLR) family, which functions as a pattern recognition receptor in innate immunity. It is characterized by a conserved tripartite structure: a pyrin domain (PYD) for protein interactions, a central NACHT domain for oligomerization, and leucine-rich repeats (LRRs) that sense pathogen-associated or damage-associated molecular patterns. NLRP12 is primarily expressed in immune cells, including monocytes, dendritic cells, and granulocytes.
Unlike other NLRPs that promote inflammasome assembly, NLRP12 acts as a negative regulator of inflammatory responses. It suppresses NF-κB and MAPK signaling pathways, often by interacting with ubiquitin ligases or kinases. NLRP12 also modulates microbial sensing and autophagy, influencing host-microbe interactions. Mutations in *NLRP12* are linked to autoinflammatory disorders, such as familial cold autoinflammatory syndrome (FCAS), and have been implicated in colitis, cancer, and autoimmune diseases.
NLRP12 antibodies are critical tools for studying its expression, localization, and functional roles. They enable detection of NLRP12 in Western blotting, immunohistochemistry, and flow cytometry. Research using these antibodies has elucidated NLRP12's dual role in inflammation and immune tolerance, highlighting its potential as a therapeutic target. However, challenges remain in standardizing antibody specificity across experimental models, necessitating careful validation for accurate interpretation in mechanistic or clinical studies.
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