| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968 |
| Entrez GeneID | 815 |
| WB Predicted band size | 54.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CAMK2A (CAMK2 alpha) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-478 amino acids from the C-terminal region of human CAMK2A (CAMK2 alpha). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于CAMK2α抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*"Distinct spatiotemporal expression of COMT and CAMK2A in human brain development: implications for synaptic plasticity"*
**作者**：Bayer KU, et al.
**摘要**：该研究通过生成特异性识别CAMK2α亚型的抗体，验证了其在人脑发育不同阶段的表达模式。抗体在免疫印迹(Western blot)和免疫组化中表现出高特异性，揭示了CAMK2α在突触可塑性中的时空表达差异。
2. **文献名称**：*"Antibody validation for neurodegenerative disease-associated proteins: CAMK2A as a case study"*
**作者**：Zhou Y, et al.
**摘要**：文章系统评估了多种市售CAMK2A抗体的特异性，发现部分抗体存在交叉反应(如与CAMK2β亚型)。通过敲除实验和肽段竞争验证，推荐了适用于脑组织切片和蛋白质组学分析的可靠抗体。
3. **文献名称**：*"Phospho-specific antibodies reveal spatiotemporal activation of CAMK2α in hippocampal neurons"*
**作者**：Coultrap SJ, Bayer KU
**摘要**：研究开发了针对CAMK2α Thr286自磷酸化位点的抗体，证实其在钙信号激活后特异性识别磷酸化CAMK2α，并应用于活体神经元成像，揭示了该激酶在长时程增强(LTP)中的动态激活过程。
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以上文献涵盖了抗体特异性验证、亚型区分及磷酸化状态检测等关键应用场景，可为基础研究和临床检测提供参考。
The CAMK2A (Calcium/Calmodulin-Dependent Protein Kinase II Alpha) antibody is a key tool for studying the function and regulation of the CAMK2α protein, a central player in calcium-mediated signaling pathways. CAMK2A is a multifunctional serine/threonine kinase highly expressed in the central nervous system, where it regulates synaptic plasticity, neurotransmitter release, and long-term potentiation (LTP), processes critical for learning and memory. The enzyme exists as a holoenzyme composed of α and β subunits, with the α isoform (encoded by the CAMK2A gene) being predominant in neurons. Its activation depends on calcium influx, which triggers binding to calmodulin, autophosphorylation, and subsequent downstream signaling. Dysregulation of CAMK2A has been implicated in neurological disorders, including Alzheimer’s disease, epilepsy, and autism spectrum disorders.
CAMK2A antibodies are widely used in neuroscience research to detect protein expression, phosphorylation status, and subcellular localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific antibodies may distinguish between inactive and active (autophosphorylated) forms, aiding in studies of kinase dynamics. Validation often includes knockout controls or siRNA-mediated silencing to confirm specificity. Researchers also utilize these antibodies to explore CAMK2A's role in disease models or drug responses. When selecting a CAMK2A antibody, considerations include species reactivity, application compatibility, and epitope recognition, as splice variants or post-translational modifications may affect detection. Proper controls are essential to ensure accurate interpretation in experimental contexts.
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