Rabbit Polyclonal Phospho-HUMAN-PIK3C2B(Y228) Antibody

The Phospho-HUMAN-PIK3C2B(Y228) antibody is a specialized tool designed to detect the phosphorylated form of the tyrosine 228 residue in the PIK3C2B protein, a member of the class II phosphatidylinositol 3-kinase (PI3K) family. PIK3C2B plays a critical role in intracellular signaling, regulating processes such as cell proliferation, survival, vesicular trafficking, and glucose metabolism. Phosphorylation at Y228 is implicated in modulating the enzyme's catalytic activity, subcellular localization, or interactions with downstream effectors, though its precise functional significance remains under investigation. This antibody is commonly used in research to study PI3K signaling dynamics, particularly in contexts like cancer, metabolic disorders, or cardiovascular diseases, where aberrant PI3K activity is often observed. It enables the detection of phosphorylation-dependent activation or regulation of PIK3C2B via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Validated specificity for the phosphorylated Y228 epitope is essential, as cross-reactivity with other phospho-tyrosine residues or PI3K isoforms could compromise data accuracy. Researchers utilize this antibody to explore therapeutic targets or biomarkers linked to dysregulated PI3K pathways, contributing to mechanistic insights into disease progression and treatment responses.