Rabbit Polyclonal TIFA(N-term) Antibody

The TIFA (TRAF-interacting protein with a Forkhead-associated domain) antibody, specifically targeting its N-terminal region, is a critical tool for studying the role of TIFA in innate immune signaling. TIFA functions as a scaffold protein that activates inflammatory responses upon sensing pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharides (LPS). Upon activation, TIFA undergoes oligomerization via its N-terminal Forkhead-associated (FHA) domain, forming signaling platforms that recruit TRAF6 and trigger downstream NF-κB and MAPK pathways, leading to pro-inflammatory cytokine production. The N-term-specific antibody enables detection of TIFA’s expression, localization, and oligomerization status in various experimental models, including Western blotting, immunofluorescence, and immunoprecipitation. Researchers utilize this antibody to investigate TIFA’s involvement in diseases like cancer, inflammation, and infections, where dysregulated immune signaling is implicated. Its specificity for the N-terminal region ensures recognition of full-length TIFA isoforms, avoiding cross-reactivity with truncated variants. Developed typically in rabbit or mouse hosts, the antibody’s applications extend to elucidating TIFA’s post-translational modifications and interactions with partners like TRAF6 or ALPK1 kinase. Overall, the TIFA (N-term) antibody is indispensable for dissecting molecular mechanisms linking innate immunity to pathological conditions.