Rabbit Polyclonal SMPD3(N-term) Antibody

The SMPD3 (N-term) antibody is designed to target the N-terminal region of sphingomyelin phosphodiesterase 3 (SMPD3), a key enzyme in sphingolipid metabolism. SMPD3. also known as neutral sphingomyelinase 2 (nSMase2), catalyzes the hydrolysis of sphingomyelin to ceramide and phosphocholine, regulating cellular processes like apoptosis, inflammation, and membrane trafficking. Its N-terminal domain is critical for enzymatic activity and protein-protein interactions. Dysregulation of SMPD3 is linked to metabolic disorders, neurodegenerative diseases, and cancer. The antibody is widely used in research to study SMPD3 expression, localization, and function in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. By specifically recognizing the N-terminal epitope, this antibody helps distinguish SMPD3 from other sphingomyelinase isoforms (e.g., SMPD1 or SMPD2), ensuring assay specificity. It has been instrumental in elucidating SMPD3's role in lipid raft signaling, exosome formation, and stress responses. Validated applications include detecting SMPD3 in liver, brain, and cancer models, aiding investigations into its therapeutic potential for conditions like non-alcoholic fatty liver disease (NAFLD) or Alzheimer’s. Proper controls (e.g., knockout samples) are recommended to confirm signal specificity.