Rabbit Polyclonal HIST3H2A(N-term) Antibody

The HIST3H2A (N-term) antibody specifically targets the N-terminal region of histone H3.2. encoded by the HIST3H2A gene (also known as H3F3A). Histone H3.2 is a replication-dependent histone variant integral to nucleosome structure, playing a critical role in chromatin compaction and epigenetic regulation. The N-terminal tail of histone H3 undergoes post-translational modifications (e.g., acetylation, methylation) that modulate chromatin accessibility and gene expression. Antibodies against the HIST3H2A N-terminus are commonly used to investigate histone dynamics, chromatin remodeling, and epigenetic mechanisms in cellular processes such as DNA repair, replication, and transcriptional regulation. They are widely applied in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). This antibody typically recognizes the unmodified or modified N-terminal epitopes, depending on its design, making it valuable for studying histone modification patterns linked to development, cancer, and other diseases. Validation often includes specificity testing in knockout models or peptide blocking assays to ensure accurate detection. Raised in hosts like rabbit or mouse, it serves as a key tool in epigenetics research, aiding in the exploration of histone-related pathways and therapeutic targets.