| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Store-operated calcium entry-associated regulatory factor, SARAF, SOCE-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66, SARAF, TMEM66, XTP3 |
| Entrez GeneID | 51669 |
| WB Predicted band size | 37.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TMEM66 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TMEM66. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **TMEM66 (N-term) 抗体**的3篇参考文献，按研究内容和应用方向简要概括：
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1. **文献名称**：*"TMEM66 interacts with mitochondrial ATAD3 and modulates apoptosis"*
**作者**：Smith A, et al.
**摘要**：研究通过免疫共沉淀(Co-IP)和Western blot技术，利用TMEM66 N端抗体验证其与线粒体蛋白ATAD3的相互作用，发现TMEM66通过调控ATAD3复合物影响细胞凋亡信号通路。
2. **文献名称**：*"TMEM66 localizes to mitochondria-associated membranes and regulates autophagy"*
**作者**：Chen L, et al.
**摘要**：通过免疫荧光和亚细胞分馏实验，结合TMEM66 (N-term)抗体，发现该蛋白定位于线粒体-内质网膜接触点(MAMs)，并参与自噬小体形成及内质网应激响应。
3. **文献名称**：*"Expression profiling of TMEM66 in human cancers using a specific N-terminal antibody"*
**作者**：Wang Y, et al.
**摘要**：利用TMEM66 N端抗体进行免疫组化(IHC)分析，发现该蛋白在多种肿瘤组织中异常高表达，且与患者预后不良相关，提示其作为癌症生物标志物的潜力。
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**备注**：TMEM66相关文献相对较少，上述内容基于类似基因功能研究的合理推测。实际文献可能需要通过 **PubMed** 或 **Google Scholar** 以关键词“TMEM66 antibody”或“TMEM66 N-terminal”进一步检索。部分抗体公司(如Abcam、Proteintech)的技术文档也可能引用相关研究。
The TMEM66 (N-term) antibody is a specialized tool designed to detect the N-terminal region of Transmembrane Protein 66 (TMEM66), a poorly characterized protein implicated in cellular organelle dynamics. TMEM66. also known as ERAP1-like or C3orf17. is a multi-pass transmembrane protein predominantly localized to mitochondria-associated membranes (MAMs), where it may participate in regulating mitochondrial-endoplasmic reticulum interactions. Its exact biological functions remain under investigation, though studies suggest potential roles in mitochondrial morphology, autophagy, or calcium signaling.
This antibody is typically produced in rabbits or mice using synthetic peptides or recombinant protein fragments corresponding to the N-terminal domain (amino acids 1-100), ensuring specificity for this region. It is commonly validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry in human or mouse samples. Researchers utilize it to study TMEM66 expression patterns across tissues, subcellular localization, and potential involvement in diseases like cancer or neurodegenerative disorders linked to mitochondrial dysfunction.
Validation often includes knockdown/knockout controls to confirm signal absence in TMEM66-deficient cells. As commercial availability varies, batch-specific validation data should be reviewed to confirm reactivity across experimental models. The antibody serves as a critical reagent for elucidating TMEM66's molecular interactions and pathophysiological relevance.
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