| WB | 咨询技术 | Mouse, Rat |
| IF | 咨询技术 | Mouse, Rat |
| IHC | 咨询技术 | Mouse, Rat |
| ICC | 技术咨询 | Mouse, Rat |
| FCM | 咨询技术 | Mouse, Rat |
| Elisa | 咨询技术 | Mouse, Rat |
| Aliases | Patatin-like phospholipase domain-containing protein 3, Acylglycerol O-acyltransferase, 231-, Adiponutrin, Calcium-independent phospholipase A2-epsilon, iPLA2-epsilon, Pnpla3, Adpn |
| Entrez GeneID | 116939 |
| WB Predicted band size | 45.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, Rat |
| Immunogen | This Mouse Pnpla3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of mouse Pnpla3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于小鼠Pnpla3抗体的相关文献示例(内容基于领域常见研究方向模拟，建议通过学术数据库验证)：
1. **"Characterization of Pnpla3 expression in murine hepatic steatosis models using a novel monoclonal antibody"**
*Authors: Smith A, et al.*
摘要：研究开发了针对小鼠Pnpla3蛋白的单克隆抗体，验证其在Western blot和免疫组化中的特异性，发现其在脂质代谢异常小鼠肝脏中表达显著上调。
2. **"Antibody-based validation of Pnpla3 knockout mice reveals compensatory lipid metabolic pathways"**
*Authors: Tanaka K, et al.*
摘要：通过抗体验证Pnpla3基因敲除小鼠模型的蛋白缺失，发现Pnpla3缺失导致肝脏甘油三酯积累减少，提示其在小鼠脂代谢中的调控作用。
3. **"Tissue-specific distribution of Pnpla3 in mouse metabolic organs detected by immunohistochemistry"**
*Authors: Lee JH, et al.*
摘要：利用特异性抗体分析Pnpla3在小鼠肝脏、脂肪组织等器官的表达分布，揭示其在高脂饮食诱导的肥胖模型中的亚细胞定位变化。
4. **"Development of a phospho-specific antibody for murine Pnpla3 to study post-translational modifications"**
*Authors: Müller R, et al.*
摘要：报道了一种针对小鼠Pnpla3磷酸化位点的抗体，证实胰岛素抵抗模型中Pnpla3的Ser-47位点磷酸化水平升高，提示翻译后修饰的功能意义。
**注意**：上述文献为模拟示例，实际文献需通过PubMed或Google Scholar以关键词“Pnpla3 mouse antibody”或“Pnpla3 immunohistochemistry murine”检索。部分研究可能使用跨物种反应性抗体，需具体查阅抗体验证数据。
**Background of Mouse PNPLA3 Antibody**
Patatin-like phospholipase domain-containing protein 3 (PNPLA3), also known as adiponutrin, is a lipid-metabolizing enzyme encoded by the *Pnpla3* gene. It is primarily expressed in the liver and adipose tissue, playing a role in triglyceride hydrolysis and lipid droplet regulation. Mouse PNPLA3 shares functional and structural homology with human PNPLA3. making murine models critical for studying its metabolic roles.
The PNPLA3 I148M variant is strongly associated with human non-alcoholic fatty liver disease (NAFLD) and liver fibrosis. Mouse *Pnpla3* antibodies are essential tools for detecting protein expression, localization, and post-translational modifications in experimental models. These antibodies enable researchers to investigate PNPLA3's role in lipid metabolism, hepatic steatosis, and disease progression.
Antibodies targeting mouse PNPLA3 are typically generated using immunogens derived from recombinant protein fragments or specific epitopes. Validated applications include Western blotting, immunohistochemistry, and immunofluorescence. Their specificity is confirmed using knockout controls or siRNA-mediated knockdown.
Research utilizing mouse PNPLA3 antibodies has advanced understanding of NAFLD pathogenesis, genetic susceptibility, and potential therapeutic targets. Studies often combine these antibodies with *Pnpla3*-knockout or transgenic mice to dissect molecular mechanisms underlying lipid dysregulation and liver injury.
In summary, mouse PNPLA3 antibodies are pivotal for exploring the enzyme's biological functions and its implications in metabolic disorders, bridging insights from murine models to human disease.
×
