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Rabbit Polyclonal ECAT1(N-term) Antibody

  • 中文名: ECAT1 (N-term)抗体
  • 别    名: KHDC3-like protein, ES cell-associated transcript 1 protein, KHDC3L, C6orf221, ECAT1
货号: IPDX34997
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesKHDC3-like protein, ES cell-associated transcript 1 protein, KHDC3L, C6orf221, ECAT1
Entrez GeneID154288
WB Predicted band size24.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human ECAT1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于 **ECAT1 (N-term) 抗体** 的参考文献示例(内容为模拟,仅供参考):

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1. **"ECAT1 as a Novel Pluripotency Marker in Human Embryonic Stem Cells"**

*Authors: Yamaguchi S, Kimura H, et al.*

**摘要**:本研究利用针对 ECAT1 N端结构域的多克隆抗体,通过免疫印迹和免疫荧光技术验证了 ECAT1 在人胚胎干细胞中的特异性表达。结果表明,ECAT1 与 OCT4、NANOG 共定位,提示其在维持多能性中的潜在作用。

2. **"Development and Validation of ECAT1-Specific Antibodies for Epigenetic Studies"**

*Authors: Tanaka K, Saitou M.*

**摘要**:文章报道了一种针对 ECAT1 蛋白 N端的高特异性单克隆抗体的开发,并通过 ChIP-seq 技术证实其可用于染色质免疫沉淀。该抗体在分析 ECAT1 与染色质修饰复合物的相互作用中表现出高灵敏度。

3. **"Dynamic Expression of ECAT1 During Early Embryogenesis in Mice"**

*Authors: Zhou Y, Zhang Y.*

**摘要**:通过 ECAT1 N端抗体的免疫组化分析,揭示了 ECAT1 在小鼠早期胚胎中的时空表达模式。研究发现 ECAT1 在囊胚内细胞团中高表达,并在分化过程中显著下调,提示其与细胞命运决定相关。

4. **"Functional Analysis of ECAT1 in Reprogramming Somatic Cells to Pluripotency"**

*Authors: Müller C, Schöler HR.*

**摘要**:利用 ECAT1 N端抗体阻断实验,研究证实 ECAT1 在体细胞重编程为诱导多能干细胞(iPSC)中的必要性。抗体介导的功能抑制导致多能性基因网络失调,进一步支持 ECAT1 的核心调控作用。

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**备注**:以上文献为示例,实际研究中 ECAT1 可能以别名(如 **DPPA5**)出现,建议结合具体基因名或抗体编号在 PubMed 等平台进一步检索。

背景信息

The ECAT1 (N-term) antibody is a tool for detecting ECAT1 (ESG1/DPPA5), a protein encoded by the *DPPA5* gene, which belongs to the developmental pluripotency-associated (DPPA) family. ECAT1 is highly expressed in embryonic stem cells (ESCs) and primordial germ cells, playing a critical role in maintaining pluripotency and self-renewal by regulating chromatin states and epigenetic modifications. It is downregulated during differentiation, making it a marker of undifferentiated pluripotent cells. The antibody specifically targets the N-terminal region of ECAT1. enabling researchers to distinguish it from homologous proteins. Validated in assays like Western blotting, immunofluorescence, and flow cytometry, it aids in studying stem cell biology, reprogramming mechanisms, and cancer research, where ECAT1 overexpression is linked to tumor-initiating cells. Host species (e.g., rabbit) and validation details (e.g., knockout controls) vary by vendor. Its applications span regenerative medicine, early development, and oncogenesis.

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