| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | N-acetyltransferase ESCO2, 231-, Establishment of cohesion 1 homolog 2, ECO1 homolog 2, ESCO2 |
| Entrez GeneID | 157570 |
| WB Predicted band size | 68.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ESCO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-161 amino acids from the Central region of human ESCO2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ESCO2抗体的文献信息，供参考：
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1. **文献名称**：Mutations in ESCO2 cause Roberts syndrome
**作者**：Vega H, et al.
**摘要**：该研究首次将ESCO2基因突变与Roberts综合征相关联，利用ESCO2抗体进行Western blot分析，发现患者细胞中ESCO2蛋白表达显著降低，揭示了该基因在姐妹染色单体结合中的关键作用。
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2. **文献名称**：Roberts syndrome is caused by mutations in ESCO2. a human homolog of yeast ECO1
**作者**：Schule B, et al.
**摘要**：研究通过ESCO2抗体检测患者成纤维细胞，证实突变导致蛋白功能丧失，阐明了ESCO2在DNA修复和染色体稳定性中的机制，为表型-基因型关联提供了依据。
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3. **文献名称**：Cellular and biochemical analysis of Roberts syndrome cell lines
**作者**：Gordillo M, et al.
**摘要**：通过免疫荧光技术结合ESCO2抗体，观察到患者细胞中异常染色体分离现象，进一步支持ESCO2缺失导致姐妹染色单体黏附缺陷，引发发育畸形。
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**备注**：以上文献均围绕ESCO2在遗传疾病中的应用，抗体主要用于蛋白表达检测及功能研究。如需具体文献链接或补充，可进一步提供详细信息。
ESCO2 (Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2) is a key protein involved in sister chromatid cohesion, a critical process during cell division that ensures proper chromosome segregation. It belongs to the ESCO family of acetyltransferases and functions by acetylating cohesin complex components, particularly SMC3. to stabilize chromatid attachment after DNA replication. This activity is essential for maintaining genomic stability and preventing chromosomal abnormalities.
ESCO2 antibodies are laboratory tools developed to detect and study the expression, localization, and function of the ESCO2 protein. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Research involving ESCO2 antibodies has been pivotal in understanding its role in developmental disorders, particularly Roberts syndrome (RBS), a rare genetic condition caused by mutations in the ESCO2 gene. Patients with RBS exhibit growth defects, craniofacial abnormalities, and limb malformations, highlighting ESCO2's importance in embryonic development.
Additionally, ESCO2 antibodies aid in exploring its interactions with other cohesion-related proteins and its broader implications in DNA repair, replication fidelity, and cancer biology. Dysregulation of ESCO2 has been linked to chromosomal instability in tumors, making it a potential biomarker or therapeutic target. Commercial ESCO2 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown to ensure reliable experimental results. Ongoing studies continue to unravel its regulatory mechanisms and disease associations.
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