| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5 HT 7; 5 HT X; 5-HT-7; 5-HT-X; 5-HT7; 5HT7; htr7;;5 HT7 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 5 HT7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于GHRH (N-term)抗体的3篇参考文献示例(注：内容为模拟，实际文献需通过学术数据库验证)：
---
1. **文献名称**: *Production and characterization of a specific antibody against the N-terminal region of human growth hormone-releasing hormone*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对人GHRH N端(1-29氨基酸)的多克隆抗体，通过ELISA和免疫印迹验证其特异性。该抗体成功应用于检测人垂体组织和血浆中的GHRH表达，为研究生长激素分泌调控提供了工具。
---
2. **文献名称**: *Immunohistochemical localization of GHRH in the rat hypothalamus using an N-terminal-specific antibody*
**作者**: Tanaka K, et al.
**摘要**: 作者利用GHRH (N-term)单克隆抗体对大鼠下丘脑进行免疫组化分析，发现GHRH神经元主要分布于弓状核。该抗体的高特异性支持了GHRH在神经内分泌调控中的定位研究。
---
3. **文献名称**: *GHRH expression in pancreatic neuroendocrine tumors detected by a novel N-terminal antibody*
**作者**: Müller B, et al.
**摘要**: 研究采用新型GHRH-N端抗体检测胰腺神经内分泌肿瘤组织，发现部分肿瘤存在GHRH异常表达，提示其可能通过自分泌机制促进肿瘤生长，为靶向治疗提供潜在方向。
---
如需实际文献，建议在PubMed或Google Scholar中搜索关键词：**"GHRH N-terminal antibody"** 或 **"GHRH antibody characterization"**，并筛选涉及抗体开发、验证或应用的论文。
Growth hormone-releasing hormone (GHRH) is a hypothalamic neuropeptide critical for regulating the synthesis and secretion of growth hormone (GH) from the anterior pituitary gland. Produced as part of a larger precursor protein (preproGHRH), mature GHRH is a 44-amino acid peptide that binds to GHRH receptors on pituitary somatotrophs, activating cAMP signaling to stimulate GH release. The N-terminal region of GHRH (approximately residues 1-29) is essential for its biological activity, as it contains the receptor-binding domain required for initiating downstream hormonal cascades.
Antibodies targeting the N-terminal region of GHRH (GHRH N-term antibodies) are valuable tools for studying GHRH expression, localization, and function in both physiological and pathological contexts. These antibodies are commonly used in techniques such as immunohistochemistry, Western blotting, and ELISA to detect GHRH in tissue samples, cell lysates, or biological fluids. They help researchers investigate GHRH dynamics in conditions like growth hormone deficiency, acromegaly, or neuroendocrine tumors. Additionally, such antibodies aid in exploring the role of GHRH in extrahypothalamic tissues, where it may influence cell proliferation, metabolism, or immune responses. Specificity for the N-terminal region ensures recognition of bioactive GHRH fragments, distinguishing them from inactive degradation products or related peptides. Validation using knockout models or peptide-blocking assays is critical to confirm antibody reliability in experimental settings.
×
