| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Uncharacterized protein C2orf73, C2orf73 |
| Entrez GeneID | 129852 |
| WB Predicted band size | 32.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This C2orf73 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-35 amino acids from the N-terminal region of human C2orf73. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于公开文献的模拟示例(请注意，C2orf73研究较少，实际文献可能需要通过数据库验证)：
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1. **文献名称**: *C2orf73 interacts with mitochondrial proteins and regulates energy metabolism in cancer cells*
**作者**: Smith A, et al. (2021)
**摘要**: 本研究利用C2orf73 N端抗体进行免疫沉淀和Western blot分析，发现C2orf73与线粒体复合物组分相互作用，敲低该基因导致癌细胞ATP生成减少，提示其在代谢调控中的作用。
2. **文献名称**: *Developmental expression profiling of C2orf73 in mouse tissues*
**作者**: Lee H, et al. (2019)
**摘要**: 通过C2orf73 N端抗体进行免疫组化，发现该蛋白在小鼠胚胎发育阶段高表达于神经组织和心脏，提示其可能参与器官形成。
3. **文献名称**: *A novel C2orf73 mutation linked to intellectual disability: Functional characterization*
**作者**: Zhang Y, et al. (2020)
**摘要**: 研究在智力障碍患者中发现C2orf73突变，使用N端抗体验证患者细胞中蛋白表达水平下降，并通过体外实验证明突变破坏蛋白稳定性。
4. **文献名称**: *Antibody validation for C2orf73: Specificity and applications in IHC/IF*
**作者**: BioTech Co. (商业抗体技术报告，非同行评审)
**摘要**: 该报告验证了C2orf73 N端抗体的特异性，显示其在免疫荧光(IF)和福尔马林固定组织中的有效性，并提供了多种细胞系的阳性/阴性对照数据。
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**备注**：以上为模拟内容，实际文献需通过PubMed、Google Scholar或抗体供应商(如Abcam、Novus产品说明书)提供的参考文献检索。建议使用关键词“C2orf73 antibody”或“C2orf73 AND (N-terminal OR epitope)”进一步筛选。
The C2orf73 (N-term) antibody is a research tool designed to target the N-terminal region of the protein encoded by the C2orf73 gene (Chromosome 2 Open Reading Frame 73), also known as CYTLER. This gene is evolutionarily conserved and expressed in various tissues, though its precise biological function remains poorly characterized. Preliminary studies suggest potential roles in ciliogenesis, cellular differentiation, or signaling pathways, with emerging links to ciliopathies and cancer. The encoded protein is predicted to be soluble and may localize to cytoplasmic or ciliary compartments.
The C2orf73 (N-term) antibody is typically developed using synthetic peptides or recombinant protein fragments corresponding to the N-terminal sequence, enabling detection of endogenous protein levels in applications like Western blotting, immunofluorescence, or immunohistochemistry. Its validation often includes knockdown/knout controls to confirm specificity. Researchers use this antibody to explore C2orf73 expression patterns, subcellular localization, and interactions in disease models, particularly in contexts involving ciliary dysfunction or tumorigenesis.
Due to limited functional data, studies employing this antibody often aim to clarify C2orf73’s mechanistic role. Commercial availability varies, and batch-specific validation is recommended. Ongoing research seeks to define its molecular interactions and therapeutic relevance, positioning the antibody as a critical reagent for unraveling C2orf73’s contributions to cellular physiology and pathology.
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