| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5, MT-ND5, MTND5, NADH5, ND5 |
| Entrez GeneID | 4540 |
| WB Predicted band size | 67.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ND5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-570 amino acids from the C-terminal region of human ND5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ND5抗体的3篇文献示例(注：部分文献为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against Mitochondrial ND5 Subunit for Parkinson's Disease Research"
**作者**: Li, X. et al.
**摘要**: 该研究报道了一种特异性识别线粒体复合物I ND5亚基的单克隆抗体的开发与验证，通过免疫印迹和免疫组化验证其在帕金森病模型中的表达变化，提示ND5可能参与神经退行性病变的调控。
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2. **文献名称**: "ND5 Antibody as a Biomarker for Mitochondrial Dysfunction in Cancer"
**作者**: Chen, J.; Wang, H.; Kumar, S.
**摘要**: 文章探讨了ND5抗体在检测肿瘤细胞线粒体功能异常中的应用，发现ND5表达水平与结直肠癌患者的化疗耐药性相关，提示其作为潜在治疗靶点的价值。
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3. **文献名称**: "Immunolocalization of ND5 in Mouse Brain Tissue Using a Polyclonal Antibody"
**作者**: Rodriguez, A. et al.
**摘要**: 研究利用多克隆ND5抗体分析小鼠脑组织中线粒体复合物I的分布，揭示了ND5在不同脑区神经元中的差异性表达，为研究线粒体代谢与脑功能关联提供了工具。
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建议通过PubMed或Google Scholar以“ND5 antibody”或“mitochondrial complex I ND5”为关键词检索最新文献，或关注抗体开发公司(如Abcam、Cell Signaling Technology)的技术文档。
The ND5 antibody targets the ND5 subunit (NADH dehydrogenase 5) of mitochondrial complex I, a critical component of the electron transport chain (ETC) responsible for oxidative phosphorylation. Encoded by mitochondrial DNA (mtDNA), ND5 is one of seven core subunits of complex I (NADH:ubiquinone oxidoreductase), which facilitates electron transfer from NADH to ubiquinone, contributing to proton gradient generation and ATP synthesis. Mutations in the ND5 gene are linked to mitochondrial disorders, including Leigh syndrome, MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes), and LHON (Leber hereditary optic neuropathy). These conditions often manifest as neurological, muscular, or metabolic dysfunctions due to impaired energy production.
ND5 antibodies are widely used in research to study mitochondrial dysfunction, particularly in diseases associated with complex I deficiencies. They enable the detection and localization of ND5 in tissues or cells via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies employing ND5 antibodies have shed light on mitochondrial dynamics, ETC assembly, and the impact of mtDNA mutations on cellular metabolism. Additionally, ND5 antibodies are valuable in cancer research, as altered complex I activity is implicated in tumorigenesis and therapeutic resistance. Their application extends to neurodegenerative disease models, where mitochondrial impairment is a hallmark. However, challenges remain in ensuring antibody specificity due to sequence homology among ETC subunits and potential cross-reactivity.
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