| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PHD finger protein 21B, PHF21B, KIAA1661 |
| Entrez GeneID | 112885 |
| WB Predicted band size | 57.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PHF21B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-279 amino acids from the Central region of human PHF21B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PHF21B抗体的3篇文献示例(内容为模拟概括，部分文献信息可能需要根据实际研究调整)：
1. **文献名称**: *PHF21B as a novel biomarker in glioblastoma: Expression analysis using a validated monoclonal antibody*
**作者**: Smith A, et al.
**摘要**: 本研究通过自主研发的小鼠单克隆抗体，首次报道了PHF21B在胶质母细胞瘤中的高表达现象，并证实其与肿瘤细胞增殖和患者预后不良相关。抗体经Western blot和免疫组化验证具有高特异性。
2. **文献名称**: *The role of PHF21B in neural development revealed by CRISPR and antibody-based knockdown*
**作者**: Chen L, et al.
**摘要**: 作者利用兔多克隆PHF21B抗体结合CRISPR技术，发现PHF21B通过调控组蛋白修饰影响神经干细胞分化。抗体特异性经siRNA敲低实验验证，提示其在神经系统疾病研究中的潜在价值。
3. **文献名称**: *Proteomic screening identifies PHF21B as an epigenetic regulator interacting with HDAC complexes*
**作者**: Gupta R, et al.
**摘要**: 该研究通过免疫共沉淀(使用商品化PHF21B抗体)结合质谱分析，揭示PHF21B与HDAC1/2复合物相互作用，参与染色质重塑过程。抗体特异性通过基因敲除细胞系验证。
4. **文献名称**: *PHF21B autoantibodies as a potential biomarker in autoimmune encephalitis*
**作者**: Watanabe K, et al.
**摘要**: 首次报道在自身免疫性脑炎患者血清中检测到PHF21B自身抗体，使用重组PHF21B蛋白包被的ELISA法证实其诊断价值，提示该抗体可能成为新型神经免疫疾病标志物。
注：以上为模拟文献，实际研究中建议通过PubMed或Web of Science以"PHF21B antibody"为关键词检索最新论文，重点关注涉及抗体验证、疾病机制或诊断应用的研究。
The PHF21B (PHD Finger Protein 21B) antibody is a tool used to study the PHF21B protein, a member of the PHD-finger family involved in chromatin remodeling and transcriptional regulation. PHF21B contains a conserved plant homeodomain (PHD) zinc finger motif, which typically mediates interactions with histone post-translational modifications, suggesting roles in epigenetic regulation. While its exact biological functions remain under investigation, PHF21B is hypothesized to influence neural development, synaptic plasticity, and cellular differentiation pathways.
PHF21B antibodies are widely employed in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and interactions. Studies have linked PHF21B to neurodevelopmental disorders and cancers, with altered expression observed in gliomas and neuroblastomas. For instance, PHF21B may act as a tumor suppressor or oncogene depending on context, though mechanistic insights are still emerging.
Antibody validation often includes knockout cell line controls to confirm specificity. Commercial PHF21B antibodies are available in monoclonal and polyclonal forms, with varying host species (e.g., rabbit, mouse). Researchers prioritize antibodies validated for particular applications to ensure reproducibility. Current investigations focus on elucidating PHF21B’s role in chromatin dynamics, its partnership with histone modifiers like histone deacetylases (HDACs), and its potential as a biomarker or therapeutic target in neurological and oncological diseases.
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