| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein APCDD1, Adenomatosis polyposis coli down-regulated 1 protein, APCDD1 (HGNC:15718) |
| Entrez GeneID | 147495 |
| WB Predicted band size | 58.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This APCDD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human APCDD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于APCDD1(N-term)抗体的参考文献示例(注：以下内容为模拟生成，具体文献需通过学术数据库核实)：
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1. **文献名称**：*APCDD1 is a novel Wnt inhibitor mutated in hereditary hypotrichosis simplex*
**作者**：Shimomura Y, et al.
**摘要**：该研究首次报道APCDD1基因突变与遗传性单纯性少毛症相关，通过N端特异性抗体证实APCDD1在毛囊中的表达，并证明其作为Wnt信号通路抑制分子的功能。
2. **文献名称**：*APCDD1 interacts with β-catenin to regulate Wnt signaling in colorectal cancer*
**作者**：Zhang L, et al.
**摘要**：研究利用APCDD1(N-term)抗体进行免疫共沉淀实验，揭示APCDD1通过N端结构域与β-catenin结合，抑制结直肠癌中Wnt信号通路的异常激活。
3. **文献名称**：*Antibody validation and localization of APCDD1 in neural development*
**作者**：Tanaka K, et al.
**摘要**：文章验证了APCDD1 N端抗体的特异性，通过免疫荧光技术证明APCDD1在小鼠胚胎神经管中的动态表达模式，提示其在神经系统发育中的作用。
4. **文献名称**：*Proteomic analysis identifies APCDD1 as a biomarker for pancreatic cancer progression*
**作者**：Wang H, et al.
**摘要**：研究使用APCDD1(N-term)抗体进行Western blot和免疫组化分析，发现APCDD1在胰腺癌组织中的高表达与患者预后不良显著相关。
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**建议**：以上为模拟示例，实际文献请通过PubMed、Google Scholar等平台以关键词“APCDD1 N-terminal antibody”或“APCDD1 antibody application”检索，并优先选择近5年高被引研究。
The APCDD1 (N-term) antibody is a monoclonal or polyclonal antibody specifically designed to target the N-terminal region of the Adenomatosis Polyposis Coli Down-Regulated 1 (APCDD1) protein. APCDD1 is a transmembrane glycoprotein that acts as a negative regulator of the Wnt/β-catenin signaling pathway, playing critical roles in developmental processes, tissue homeostasis, and cancer progression. It is highly expressed in tissues such as the brain, skin, and intestine, and its dysregulation has been implicated in colorectal cancer, hereditary hypotrichosis, and neurodegenerative disorders.
This antibody is commonly utilized in research applications like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect APCDD1 expression levels and localization in cell or tissue samples. The N-terminal specificity ensures recognition of full-length APCDD1. avoiding cross-reactivity with truncated isoforms or degradation products. Validated for use in human, mouse, and rat models, it aids in studying APCDD1's functional mechanisms, including its interaction with Wnt ligands (e.g., Wnt3a, Wnt7a) and its role in modulating β-catenin-dependent transcriptional activity. Researchers also employ this antibody to explore APCDD1's involvement in hair follicle regeneration, adipogenesis, and tumor suppression pathways. Proper validation via knockout (KO) controls or siRNA knockdown is recommended to confirm signal specificity. Its application contributes to understanding diseases linked to Wnt signaling aberrations and potential therapeutic targeting.
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