关键信息
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基因名
GNG4
- 应用
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别名
GNG4;GNGT4;Guanine nucleotide-binding Protein G(I)/G(S)/G(O) subunit gamma-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50150
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表达区间
1-72aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMKEGMSNNSTTSISQARKAVEQLKMEA CMDRVKVSQAAADLLAYCEAHVREDPLIIPVPASENPFREKKFFC
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分子量
10 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GNG4, a member of the guanine nucleotide-binding protein subunit family, plays a crucial role in various cellular signaling pathways by regulating the activity of G protein-coupled receptors (GPCRs). The study of GNG4 recombinant protein has gained significance due to its implications in diverse physiological processes, including neurotransmission, immune response, and hormonal regulation. Abnormalities in GNG4 expression or function have been associated with several diseases, including cancer and neurological disorders. Researchers aim to elucidate the biological functions and underlying mechanisms of GNG4 by producing recombinant proteins for detailed structural and functional analysis. This includes examining the interactions with various GPCRs and other signaling molecules, which can provide insights into its role in health and disease. Moreover, obtaining a stable and pure form of GNG4 is essential for developing potential therapeutic strategies targeting related signaling pathways. The ongoing research focuses on optimizing expression systems and purification methods to facilitate detailed studies, aiming to contribute to the understanding of GNG4’s role in cellular signaling and pave the way for novel therapeutic interventions.
