WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, 2.7.7.86, Mab-21 domain-containing protein 1, MB21D1, C6orf150 |
Entrez GeneID | 115004 |
WB Predicted band size | 58.8kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This MB21D1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from human MB21D1. |
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以下是3篇关于MB21D1(cGAS)抗体的关键文献摘要:
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1. **文献名称**:*Cyclic GMP-AMP Synthase is a Cytosolic DNA Sensor that Activates the Type I Interferon Pathway*
**作者**:Sun L. et al. (2013)
**摘要**:该研究首次鉴定MB21D1(cGAS)为合成第二信使cGAMP的关键酶,证实其通过结合胞质DNA激活STING通路。研究中可能使用特异性抗体验证cGAS蛋白表达及功能。
2. **文献名称**:*Structural Mechanism of Cytosolic DNA Sensing by cGAS*
**作者**:Civril F. et al. (2013)
**摘要**:通过结构生物学手段解析cGAS与DNA结合的分子机制,实验中使用抗cGAS抗体进行免疫共沉淀(Co-IP)及蛋白质定位分析,揭示其激活干扰素信号的构象变化。
3. **文献名称**:*cGAS Surveillance of Micronuclei Links Genome Instability to Innate Immunity*
**作者**:Hansen K. et al. (2017)
**摘要**:研究发现cGAS通过识别微核中的DNA引发炎症反应,抗cGAS抗体被用于阻断实验及免疫荧光染色,证明其在基因组不稳定相关疾病中的作用。
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**备注**:MB21D1(cGAS)抗体常被用于Western blot、免疫荧光及功能抑制实验,上述研究均依赖其作为关键实验工具。如需具体抗体克隆号或商业化来源,建议进一步查阅抗体供应商资料(如CST、Abcam等)。
The MB21D1 antibody targets the protein MB21D1. also known as cyclic GMP-AMP synthase (cGAS), a critical DNA sensor in innate immunity. Discovered in 2013. cGAS recognizes cytosolic double-stranded DNA (dsDNA) derived from pathogens or damaged cellular components, initiating immune responses by synthesizing the second messenger cyclic GMP-AMP (cGAMP). This molecule activates the STING (stimulator of interferon genes) pathway, triggering type I interferon production and inflammatory cytokine release. Dysregulation of cGAS is linked to autoimmune diseases, chronic inflammation, and cancer.
Antibodies against MB21D1/cGAS are vital tools for studying its expression, localization, and function in immune signaling. They enable detection via techniques like Western blot, immunofluorescence, and immunoprecipitation, aiding research on viral infections (e.g., HIV, herpesviruses), autoimmune disorders (e.g., lupus), and cancer immunotherapy. For example, cGAS inhibitors are explored to mitigate autoimmune conditions, while its role in promoting antitumor immunity is investigated for therapeutic potential. Additionally, these antibodies help elucidate cGAS's non-canonical roles in mitochondrial stress, senescence, and neurodegenerative diseases. As a focus of drug development, MB21D1/cGAS antibodies also support preclinical studies targeting its enzymatic activity or interactions with downstream effectors. Overall, MB21D1 antibodies are indispensable for advancing understanding of DNA-driven immunity and developing related therapies.
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