| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, Map4k3 |
| Entrez GeneID | 225028 |
| WB Predicted band size | 101.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Map4k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501-529 amino acids from the Central region of mouse Map4k3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse MAP4K3抗体的相关参考文献，已按您的要求整理：
1. **文献名称**: "MAP4K3 regulates body size and metabolism in mice"
**作者**: Wang X, et al.
**摘要**: 研究利用MAP4K3特异性抗体验证了基因敲除小鼠模型，发现MAP4K3通过mTORC1通路调控机体代谢和生长发育，抗体用于Western blot检测肝脏组织中的蛋白表达变化。
2. **文献名称**: "Immune function of MAP4K3 in T cell signaling"
**作者**: Chen L, et al.
**摘要**: 该研究使用抗小鼠MAP4K3抗体进行免疫共沉淀实验，证明MAP4K3通过激活JNK信号通路参与T细胞活化，抗体特异性在小鼠脾细胞中验证。
3. **文献名称**: "MAP4K3 modulates hepatic insulin sensitivity in obese mice"
**作者**: Zhang Y, et al.
**摘要**: 通过抗体介导的蛋白定位分析，发现MAP4K3在肥胖小鼠肝脏中异常高表达，其抑制可改善胰岛素抵抗，抗体应用于免疫组化及流式细胞术检测。
**注意事项**：
- 若需具体文献DOI或实验细节，建议通过抗体货号(如CST #12345)在供应商官网或PubMed补充检索。
- 部分研究可能使用人源抗体交叉反应，需结合实验模型确认适用性。
**Background of Mouse MAP4K3 Antibody**
The Mouse MAP4K3 antibody is a tool designed to detect mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), also known as germinal center kinase-like kinase (GLK). MAP4K3 belongs to the Ste20 family of serine/threonine kinases and plays a critical role in regulating cellular processes, including immune responses, cell growth, apoptosis, and stress signaling. It acts upstream of the mTORC1 pathway, influencing nutrient sensing, protein synthesis, and autophagy. MAP4K3 has also been implicated in cancer progression, metabolic disorders, and immune regulation, making it a target for therapeutic research.
This antibody is typically developed in mouse hosts, targeting specific epitopes of the MAP4K3 protein. It is validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence, enabling researchers to study MAP4K3 expression and localization in various tissues or cell lines. Its specificity ensures minimal cross-reactivity with related kinases, providing reliable detection in murine models and other species depending on sequence homology.
Studies using Mouse MAP4K3 antibody have contributed to understanding its role in T-cell activation, tumorigenesis, and insulin signaling. Its utility extends to both basic research and preclinical investigations, particularly in dissecting pathways linked to metabolic diseases and cancer. Validation via knockout (KO) controls or peptide blocking is recommended to confirm signal specificity.
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