| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-lysine N-methyltransferase SETD1B, Lysine N-methyltransferase 2G, SET domain-containing protein 1B, hSET1B, SETD1B, KIAA1076, KMT2G, SET1B |
| Entrez GeneID | 23067 |
| WB Predicted band size | 212.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SET1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1806-1839 amino acids from the C-terminal region of human SET1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SET1B抗体的代表性文献示例(注：以下内容为模拟概括，实际文献请通过学术数据库验证)：
1. **"SET1B-mediated histone H3K4 methylation regulates transcriptional activation in mammalian cells"**
- 作者：Li, Y. et al.
- 摘要：本研究利用SET1B特异性抗体，通过ChIP-seq和CRISPR敲除技术，揭示SET1B在H3K4三甲基化修饰中的关键作用，并证明其通过调控靶基因转录参与细胞增殖。
2. **"SET1B deficiency impairs DNA damage response and promotes genomic instability"**
- 作者：Smith, J. & Patel, R.
- 摘要：通过免疫沉淀(IP)和Western blot分析SET1B抗体，发现SET1B与DNA修复蛋白相互作用，其缺失导致同源重组修复效率降低，增加癌细胞对放射治疗的敏感性。
3. **"Tissue-specific roles of SET1B in embryonic development and metabolic regulation"**
- 作者：Zhang, H. et al.
- 摘要：利用SET1B抗体进行组织免疫染色，发现SET1B在小鼠胚胎发育中高表达，并通过调控代谢相关基因(如PGC-1α)影响能量稳态。
4. **"SET1B cooperates with oncogenic pathways to drive leukemia progression"**
- 作者：García-Castro, M. et al.
- 摘要：通过流式细胞术和SET1B抗体检测，证明SET1B在急性髓系白血病中异常高表达，且与MYC蛋白协同促进肿瘤细胞的表观遗传重编程。
---
**提示**：实际文献需通过PubMed、Web of Science等平台以“SET1B antibody”或“SET1B function”为关键词检索，并筛选涉及抗体实验(如WB、IHC、ChIP)的研究。部分研究可能侧重SET1B功能而非抗体开发，建议结合具体应用场景进一步筛选。
The SET1B antibody is a crucial tool for studying the SET1B (SET Domain Containing 1B) protein, a histone methyltransferase within the COMPASS (Complex of Proteins Associated with Set1) family. SET1B, along with its homolog SET1A, catalyzes the trimethylation of histone H3 at lysine 4 (H3K4me3), a key epigenetic modification associated with active gene transcription. While SET1A and SET1B share functional redundancy, SET1B exhibits distinct expression patterns, with higher abundance in certain tissues like hematopoietic cells and the brain, suggesting specialized roles in development and cellular differentiation.
Research using SET1B antibodies has highlighted its involvement in regulating embryonic development, stem cell maintenance, and hematopoiesis. Studies in knockout models reveal that SET1B deficiency leads to embryonic lethality and defects in blood cell formation, underscoring its essential role in early development. Additionally, SET1B is implicated in cancer, particularly leukemia, where dysregulated H3K4 methylation contributes to oncogenic gene expression programs.
SET1B antibodies are widely employed in techniques such as chromatin immunoprecipitation (ChIP-seq), Western blotting, and immunofluorescence to map its genomic targets, assess protein expression, and localize its activity within the nucleus. These applications advance understanding of SET1B’s interplay with other epigenetic regulators and its impact on transcriptional networks in health and disease. As aberrant histone methylation emerges as a therapeutic target, SET1B antibodies remain vital for dissecting its mechanisms in both basic and translational research contexts.
×
