| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Properdin, Complement factor P, CFP, PFC |
| Entrez GeneID | 5199 |
| WB Predicted band size | 51.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-223 amino acids from the Central region of human CFP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CFP抗体的模拟参考文献示例(注：CFP在此假设为结核分枝杆菌的CFP-10抗原，实际文献需根据具体研究领域检索)：
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1. **文献名称**：*Development of a Monoclonal Antibody against Mycobacterium tuberculosis CFP-10 for Rapid Immunodiagnosis*
**作者**：Smith A, et al.
**摘要**：研究报道了一种针对结核分枝杆菌CFP-10抗原的高特异性单克隆抗体的开发，验证了其在ELISA和侧流层析试纸条中对活动性结核病的快速检测效果，灵敏度达92%。
2. **文献名称**：*Structural Characterization of CFP Antibody Binding Epitopes Using X-ray Crystallography*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了CFP-10蛋白与单克隆抗体的复合物结构，揭示了关键抗原表位，为改进结核病血清学诊断试剂提供了分子基础。
3. **文献名称**：*CFP Antibody Cross-Reactivity in Non-Tuberculous Mycobacteria: Implications for Diagnostics*
**作者**：Gomez R, et al.
**摘要**：评估了CFP-10抗体在非结核分枝杆菌感染中的交叉反应性，指出需结合多抗原检测以提高诊断特异性，避免假阳性结果。
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**注意事项**：
- 上述文献为模拟示例，实际研究需查询PubMed、Google Scholar等平台，使用关键词如 **"CFP-10 antibody tuberculosis"** 或 **"CFTR-associated protein antibody"**(若指其他CFP类型)。
- CFP可能在不同领域有不同含义，建议明确全称(如Cystic Fibrosis Protein、Cell Fusion Protein等)以精准检索。
CFP (Cyan Fluorescent Protein) antibodies are immunological tools designed to detect and study CFP, a engineered variant of the green fluorescent protein (GFP) originally isolated from the jellyfish *Aequorea victoria*. CFP emits cyan light (~476 nm) when excited by specific wavelengths (~434 nm), making it a key member of the fluorescent protein family widely used in molecular and cellular biology. It serves as a versatile tag for visualizing protein localization, tracking dynamic cellular processes, and studying protein-protein interactions via fluorescence resonance energy transfer (FRET) when paired with compatible fluorophores like YFP.
CFP antibodies are generated by immunizing hosts (e.g., rabbits, mice) with purified recombinant CFP or its immunogenic peptides. These antibodies exhibit high specificity for CFP's unique epitopes, enabling applications such as Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry. They are particularly valuable in experiments where CFP-fused proteins are co-expressed with other fluorescently tagged molecules, allowing precise differentiation between multiple targets. Commercial CFP antibodies are available as monoclonal or polyclonal formulations, often validated for minimal cross-reactivity with GFP or other spectral variants. Researchers select these antibodies based on experimental needs, considering factors like host species compatibility, conjugation labels (e.g., HRP, fluorophores), and validation in specific assays. Their development has significantly advanced live-cell imaging and multiplexed detection in complex biological systems.
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