Rabbit Polyclonal SCAP(N-term) Antibody

The SCAP (SREBP Cleavage-Activating Protein) antibody, particularly targeting its N-terminal region, is a critical tool for studying cholesterol and lipid metabolism regulation. SCAP is an endoplasmic reticulum (ER)-resident membrane protein that acts as a cholesterol sensor and chaperone for sterol regulatory element-binding proteins (SREBPs), transcription factors controlling genes involved in cholesterol and fatty acid synthesis. Under low cholesterol conditions, SCAP escorts SREBPs from the ER to the Golgi, where SREBPs undergo proteolytic activation to promote lipid biosynthesis. When cellular cholesterol levels rise, SCAP binds to insulin-induced gene (Insig) proteins, retaining the SCAP-SREBP complex in the ER and halting lipid production. The N-terminal domain of SCAP is essential for its interaction with Insig proteins and sterol-sensing functions. Antibodies targeting this region are widely used in research to detect SCAP expression, monitor its conformational changes, and study its regulatory mechanisms in conditions like atherosclerosis, metabolic syndrome, and obesity. They enable techniques such as Western blotting, immunoprecipitation, and immunofluorescence, aiding in understanding how SCAP dysfunction contributes to lipid-related diseases or responds to therapeutic interventions targeting cholesterol homeostasis.