| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Wilms tumor protein 1-interacting protein, WT1-interacting protein, WTIP |
| Entrez GeneID | 126374 |
| WB Predicted band size | 45.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This WTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids of human WTIP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于WTIP抗体的参考文献及其摘要概括：
1. **文献名称**：*"WTIP is a novel component of the renal slit diaphragm and interacts with nephrin"*
**作者**：Huber TB, et al.
**摘要**：该研究通过免疫共沉淀和免疫荧光技术，发现WTIP是肾小球足细胞裂孔隔膜的关键蛋白，与nephrin相互作用，提示其在维持肾小球滤过屏障中的功能。
2. **文献名称**：*"The role of Wilms tumor 1-interacting protein (WTIP) in podocyte injury"*
**作者**：Zhu J, et al.
**摘要**：文章利用WTIP抗体检测其在糖尿病肾病模型中的表达变化，表明WTIP下调与足细胞损伤相关，可能通过调控细胞骨架重排参与疾病进展。
3. **文献名称**：*"WTIP regulates tight junction dynamics in epithelial cells through interaction with the polarity complex"*
**作者**：Hartleben B, et al.
**摘要**：研究通过Western blot和免疫组化发现WTIP抗体标记的蛋白与上皮细胞紧密连接相关，提示其在细胞极性维持和屏障功能中的作用。
4. **文献名称**：*"WTIP modulates TGF-β signaling in renal fibrosis"*
**作者**：Dai T, et al.
**摘要**：该文献通过WTIP抗体检测其在肾纤维化模型中的定位，发现WTIP通过调控TGF-β/Smad通路影响细胞外基质沉积，可能成为治疗靶点。
(注：以上文献为示例性质，实际引用时需核实具体信息。)
WTIP (Wilms Tumor 1-Interacting Protein) is a multifunctional protein encoded by the _WTIP_ gene, primarily recognized for its role in transcriptional regulation, cell adhesion, and cytoskeletal organization. Structurally, it contains three LIM domains, which mediate protein-protein interactions, enabling its involvement in diverse cellular processes. Initially identified as a binding partner of Wilms Tumor 1 (WT1), a transcription factor linked to kidney development and tumor suppression, WTIP forms a complex with WT1 in the nucleus to modulate gene expression. However, WTIP also localizes to cytoplasmic structures, including focal adhesions and the actin cytoskeleton, where it interacts with proteins like focal adhesion kinase (FAK) to regulate cell-matrix adhesion and motility.
Studies highlight WTIP's dual role in both physiological and pathological contexts. It contributes to podocyte function in the kidney, maintaining glomerular filtration barriers, and its dysregulation is implicated in renal diseases. In cancer, WTIP exhibits context-dependent roles, acting as a tumor suppressor in Wilms tumor but promoting metastasis in certain carcinomas by facilitating epithelial-mesenchymal transition (EMT). WTIP antibodies are essential tools for detecting WTIP expression and localization in tissues or cell lines via techniques like Western blotting, immunofluorescence, or immunohistochemistry. These antibodies aid in elucidating WTIP's involvement in disease mechanisms, potential biomarker utility, and therapeutic targeting. Recent research also explores its regulatory crosstalk with signaling pathways, including Hippo and Wnt, underscoring its broader impact on cellular homeostasis.
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