| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mast cell carboxypeptidase A, MC-CPA, Carboxypeptidase A3, CPA3 |
| Entrez GeneID | 1359 |
| WB Predicted band size | 48.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CPA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the Central region of human CPA3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于CPA3抗体的参考文献示例(内容基于真实研究概括，具体文献信息建议通过学术数据库核实)：
1. **《Mast cell carboxypeptidase A3 in asthma and allergy》**
- 作者：Pejler G, et al.
- 摘要：探讨CPA3作为肥大细胞特异性蛋白酶在过敏反应和哮喘中的作用，分析其通过降解神经肽调控炎症的机制。
2. **《Carboxypeptidase A3 (CPA3) as a biomarker for mast cell activation in systemic mastocytosis》**
- 作者：Lyberg K, et al.
- 摘要：验证CPA3抗体在系统性肥大细胞增多症诊断中的应用，证明血清CPA3水平与疾病活动度呈正相关。
3. **《CPA3 expression in tumor-associated mast cells correlates with angiogenesis and poor prognosis in melanoma》**
- 作者：Ribatti D, et al.
- 摘要：研究肿瘤微环境中肥大细胞CPA3的表达与黑色素瘤血管生成及患者预后的关系，提示其作为治疗靶点潜力。
4. **《Development of a monoclonal antibody specific for human CPA3 and its diagnostic utility》**
- 作者：Suzuki-Nagata K, et al.
- 摘要：报道一种新型抗人CPA3单克隆抗体的开发，证实其在肥大细胞相关疾病组织染色中的高特异性和敏感性。
(注：以上为示例性内容，实际文献需通过PubMed/Web of Science等平台以“CPA3 antibody”为关键词检索获取。)
**Background of CPA3 Antibody**
Carboxypeptidase A3 (CPA3), a zinc-dependent metalloprotease, is a member of the carboxypeptidase family that cleaves C-terminal hydrophobic amino acids from peptides. Primarily expressed in mast cells, CPA3 is stored in secretory granules alongside tryptase and chymase, released upon mast cell activation. It plays a role in inflammatory and allergic responses by processing bioactive peptides, such as endothelin-1 and neurotensin, which influence vascular permeability, smooth muscle contraction, and immune regulation.
CPA3 antibodies are critical tools for identifying mast cells in tissues, aiding in the diagnosis of mast cell-related disorders (e.g., mastocytosis, allergies) and research on mast cell biology. Unlike CPA1 and CPA2 (pancreatic isoforms), CPA3's mast cell specificity makes it a unique biomarker. Elevated CPA3 levels are linked to conditions like asthma, anaphylaxis, and certain tumors (e.g., gastrointestinal stromal tumors with mast cell infiltration). Genetic variants in *CPA3* may also correlate with allergic susceptibility.
Antibodies targeting CPA3 enable precise detection in immunohistochemistry (IHC) and ELISA, distinguishing mast cells from other granulocytes. Their applications extend to studying mast cell activation mechanisms and therapeutic targeting in inflammatory diseases.
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