| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RCC1 and BTB domain-containing protein 2, Chromosome condensation 1-like, CHC1-L, RCC1-like G exchanging factor, Regulator of chromosome condensation and BTB domain-containing protein 2, RCBTB2, CHC1L, RLG |
| Entrez GeneID | 1102 |
| WB Predicted band size | 60.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CHC1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human CHC1L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于CHC1L(N-term)抗体的参考文献示例(内容基于文献主题概括，部分为模拟示例)：
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1. **"Role of CHC1L in endocytic trafficking revealed by N-terminal specific antibody"**
*Authors: Zhang Y, et al.*
**摘要**：本研究利用针对CHC1L蛋白N端结构域的特异性抗体，揭示了其在网格蛋白介导的内吞运输中的作用。通过免疫荧光和共聚焦显微镜，作者发现CHC1L与早期内体标志物共定位，提示其参与调控膜泡运输过程。
2. **"CHC1L N-terminal antibody validates protein expression in neuronal development"**
*Authors: Lee S, et al.*
**摘要**：文章开发了一种针对CHC1L N端的高效单克隆抗体，并通过Western blot和免疫组化验证其在神经元中的表达。研究发现CHC1L在突触形成中可能通过调控囊泡循环影响神经发育。
3. **"A novel CHC1L-interacting protein identified by immunoprecipitation using N-term-specific antibody"**
*Authors: Gupta R, et al.*
**摘要**：利用CHC1L N端抗体进行免疫共沉淀实验，鉴定出多个与CHC1L相互作用的蛋白，包括AP2复合物成员。研究提出CHC1L可能通过结合内吞相关蛋白参与细胞信号转导。
4. **"Aberrant CHC1L expression in cancer cells detected by N-terminal epitope antibody"**
*Authors: Wang H, et al.*
**摘要**：通过CHC1L N端抗体检测发现，该蛋白在多种癌细胞系中表达上调，且与肿瘤转移相关。功能实验表明，CHC1L可能通过调控受体酪氨酸激酶的稳定性促进癌症进展。
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**注意**：以上文献信息为示例性概括，实际引用需根据具体研究补充。建议通过PubMed或Google Scholar以“CHC1L antibody”、“clathrin heavy chain-like 1 N-terminal”等关键词检索最新文献。部分商业抗体供应商(如Abcam、Thermo Fisher)的产品说明页也可能提供相关引用文献。
The CHC1L (N-term) antibody is a targeted reagent designed to detect the N-terminal region of Clathrin Heavy Chain 1-Like (CHC1L), a protein implicated in intracellular membrane trafficking and vesicle formation. Clathrin, a triskelion-shaped protein complex, plays a central role in endocytosis and organelle transport by assembling into lattice structures that deform membranes into coated vesicles. CHC1L shares structural homology with the canonical clathrin heavy chain (CHC) but may exhibit distinct functional roles or tissue-specific expression patterns. The N-terminal domain of CHC1L is critical for interactions with accessory proteins, such as adaptors or regulatory molecules, which mediate cargo selection and vesicle budding.
This antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study CHC1L expression, localization, and interaction networks. Researchers employ it to explore CHC1L's involvement in cellular processes like receptor internalization, synaptic vesicle recycling, or viral entry mechanisms. Its specificity for the N-terminal region helps distinguish CHC1L from other clathrin isoforms or splice variants. Validation typically includes knockout controls or siRNA-mediated silencing to confirm target specificity. Studies utilizing this antibody may shed light on diseases linked to trafficking defects, such as cancer metastasis, neurodegenerative disorders, or pathogenic infections.
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