Mouse Monoclonal SULT2B1 Antibody

The sulfotransferase 2B1 (SULT2B1) antibody is a crucial tool for studying the SULT2B1 enzyme, a member of the cytosolic sulfotransferase family involved in the sulfonation of steroids, cholesterol, and other hydroxysteroids. SULT2B1 exists as two splice variants, SULT2B1a and SULT2B1b, which differ in tissue distribution and substrate specificity. Unlike SULT2A1. which is primarily expressed in the liver, SULT2B1 isoforms are found in extrahepatic tissues, including the skin, placenta, prostate, and brain, playing roles in cholesterol homeostasis, hormone inactivation, and cell signaling. The SULT2B1 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect the enzyme’s expression and localization in various biological samples. Researchers employ this antibody to investigate SULT2B1’s involvement in pathological conditions, such as cancer progression (e.g., prostate and liver cancers), inflammatory skin disorders, and metabolic diseases. Its specificity is often validated using knockout controls or siRNA-mediated silencing to ensure accurate detection of the target isoform. Given SULT2B1’s role in modulating bioactive molecules, including oxysterols and steroid hormones, the antibody also aids in exploring therapeutic strategies targeting sulfonation pathways. Commercial SULT2B1 antibodies are typically raised against peptide sequences unique to the enzyme, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) varying by supplier. Proper validation is essential to avoid cross-reactivity with related sulfotransferases.