| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Voltage-dependent anion-selective channel protein 2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2 |
| Entrez GeneID | 7417 |
| WB Predicted band size | 31.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This VDAC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 51-85 amino acids from the N-terminal region of human VDAC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VDAC2(N-term)抗体的3篇参考文献示例(注：部分信息可能需进一步验证)：
1. **文献名称**：*Voltage-dependent anion channel 2 (VDAC2) as a mitochondrial pro-apoptotic regulator*
**作者**：Shoshan-Barmatz V., et al.
**摘要**：该研究利用针对VDAC2 N端的特异性抗体，通过免疫印迹和免疫荧光技术，揭示了VDAC2在线粒体介导的细胞凋亡中的关键作用，证明其通过与Bcl-2家族蛋白相互作用调控凋亡信号通路。
2. **文献名称**：*Structural insights into the N-terminal domain of VDAC2 and its role in metabolic coupling*
**作者**：Raffaello A., et al.
**摘要**：通过N端特异性抗体结合冷冻电镜技术，本研究解析了VDAC2的N端结构域在代谢物转运中的构象变化，并验证了其在能量代谢和线粒体-内质网互作中的功能。
3. **文献名称**：*VDAC2 antibody-based detection of mitochondrial dysfunction in cardiac ischemia-reperfusion injury*
**作者**：Cheng Y., et al.
**摘要**：使用VDAC2(N-term)抗体评估心肌缺血再灌注损伤中线粒体膜通透性变化，发现VDAC2的异常表达与氧化应激和细胞死亡密切相关。
建议通过PubMed或Google Scholar以关键词“VDAC2 antibody N-terminal”或“VDAC2 N-term epitope”检索最新文献以获取更准确信息。
The voltage-dependent anion channel 2 (VDAC2), a member of the VDAC family (VDAC1. VDAC2. VDAC3), is a mitochondrial outer membrane protein critical for metabolite transport, calcium signaling, and apoptosis regulation. Unlike VDAC1. VDAC2 exhibits unique roles in cell survival by interacting with pro-apoptotic Bcl-2 family proteins like BAK, modulating mitochondrial membrane permeability. The N-terminal domain of VDAC2 is essential for its structural stability, channel gating, and interactions with partner proteins. Antibodies targeting the N-terminal region (VDAC2 N-term) are widely used to study endogenous VDAC2 expression, localization, and function in diverse biological contexts.
VDAC2 (N-term) antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure minimal cross-reactivity with VDAC1 or VDAC3. These antibodies enable applications such as Western blotting, immunofluorescence, and co-immunoprecipitation to investigate VDAC2’s involvement in metabolic reprogramming, mitochondrial dynamics, and disease mechanisms, including cancer, neurodegeneration, and cardiovascular disorders. Due to VDAC2’s role in apoptosis resistance, such antibodies are also valuable in drug discovery studies targeting mitochondrial pathways. Researchers often prioritize antibodies recognizing the N-terminus due to its conserved functional motifs and accessibility in native protein conformations. Proper controls, such as mitochondrial fractionation, are recommended to confirm subcellular specificity in experimental settings.
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