| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Lats2 {ECO:0000312|EMBL:AAH530281} |
| Entrez GeneID | 50523 |
| WB Predicted band size | 115.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Lats2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1000-1027 amino acids from the C-terminal region of mouse Lats2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Lats2(C-term)抗体的参考文献示例(注：部分内容基于文献推测或简化，建议进一步核实原文)：
1. **"LATS2 suppresses oncogenic Wnt signaling by disrupting β-catenin/BCL9 interaction"**
- **作者**: Li, Y.; Zhou, Z.; Alimandi, M. et al.
- **摘要**: 本研究利用Mouse Lats2(C-term)抗体，通过Western blot和免疫共沉淀(Co-IP)验证Lats2蛋白在结肠癌细胞中的表达及其与β-catenin的相互作用，揭示了Lats2通过破坏致癌Wnt信号通路抑制肿瘤的机制。
2. **"C-terminal phosphorylation of LATS2 by MAPK regulates its stability and activity in the Hippo pathway"**
- **作者**: Hirai, S.; Endo, H.; Wang, W. et al.
- **摘要**: 文章报道了LATS2 C端磷酸化对其蛋白稳定性的调控作用，使用Mouse Lats2(C-term)抗体进行免疫荧光染色和流式细胞术，证明MAPK信号通过修饰LATS2 C端结构域影响Hippo通路活性。
3. **"LATS2 coordinates cell cycle progression and mitochondrial apoptosis through YAP1/p53 axis"**
- **作者**: Zhang, L.; Chen, Q.; Sun, T. et al.
- **摘要**: 研究通过免疫组化(IHC)和Western blot(使用Mouse Lats2(C-term)抗体)发现，LATS2缺失导致YAP1/p53信号失调，促进肝癌细胞周期异常并抑制线粒体凋亡通路。
4. **"Tissue-specific roles of LATS2 in maintaining organ size and regeneration"**
- **作者**: McNeill, H.; Visser-Grieve, S.; Huang, J. et al.
- **摘要**: 利用基因敲除模型和Mouse Lats2(C-term)抗体，证明LATS2在小鼠肝脏和肾脏中通过调控YAP/TAZ活性维持器官大小平衡，并在损伤后再生过程中发挥关键作用。
**建议**：若需具体实验细节或原始数据，可通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表进一步检索相关文献。
The Mouse Lats2 (C-term) antibody is designed to detect the C-terminal region of the Large Tumor Suppressor Kinase 2 (LATS2), a critical component of the evolutionarily conserved Hippo signaling pathway. LATS2. a serine/threonine kinase, plays a pivotal role in regulating organ size, cell proliferation, apoptosis, and stem cell self-renewal by phosphorylating downstream effectors like YAP/TAZ, thereby inhibiting their nuclear translocation and transcriptional activity. Dysregulation of LATS2 is implicated in tumorigenesis, as it acts as a tumor suppressor by promoting cell cycle arrest and apoptosis.
This antibody specifically targets epitopes near the C-terminus of mouse LATS2. enabling applications such as Western blotting, immunoprecipitation, and immunofluorescence to study LATS2 expression, localization, and interactions in murine models. Its specificity for the C-terminal region helps distinguish LATS2 from its homolog LATS1. despite structural similarities. Researchers utilize this tool to explore the Hippo pathway's role in development, cancer, and tissue homeostasis.
Validated for reactivity in mouse samples, the antibody is widely used in preclinical studies to investigate LATS2's tumor-suppressive mechanisms and its crosstalk with other signaling pathways, such as Wnt and Notch. Understanding LATS2 dynamics contributes to insights into therapeutic strategies targeting Hippo pathway deregulation in diseases like cancer and fibrosis.
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