关键信息
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基因名
gldA
- 应用
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别名
gldA;Hydroxyacid-oxoacid transhydrogenase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32816
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表达区间
1-370aa
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氨基酸序列
MAAERVFISP AKYVQGKNVI TKIANYLEGI GNKTVVIADE IVWKIAGHTI VNELKKGNIA AEEVVFSGEA SRNEVERIAN IARKAEAAIV IGVGGGKTLD TAKAVADELD AYIVIVPTAA STDAPTSALS VIYSDDGVFE SYRFYKKNPD LVLVDTKIIA NAPPRLLASG IADALATWVE ARSVIKSGGK TMAGGIPTIA AEAIAEKCEQ TLFKYGKLAY ESVKAKVVTP ALEAVVEANT LLSGLGFESG GLAAAHAIHN GFTALEGEIH HLTHGEKVAF GTLVQLALEE HSQQEIERYI ELYLSLDLPV TLEDIKLKDA SREDILKVAK AATAEGETIH NAFNVTADDV ADAIFAADQY AKAYKEKHRK
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The gldA gene encodes for glucose-6-phosphate dehydrogenase (G6PD), an essential enzyme involved in the pentose phosphate pathway, which is crucial for cellular metabolism and maintaining redox balance. Research on gldA recombinant proteins has gained significant attention due to their potential applications in biotechnology and medicine. G6PD plays a vital role in protecting cells from oxidative stress by generating NADPH, a reducing agent that is pivotal for biosynthetic reactions and the detoxification of reactive oxygen species (ROS). Mutations in the gldA gene can lead to G6PD deficiency, resulting in various health issues, including hemolytic anemia. By producing gldA recombinant proteins, researchers aim to better understand the enzyme's structure-function relationship, explore its regulatory mechanisms, and establish its role in different biological processes. Additionally, gldA recombinant proteins are of great interest for developing diagnostic tools and therapeutic strategies for conditions associated with G6PD deficiency. The ability to produce these proteins in large quantities through recombinant DNA technology allows for detailed biochemical characterization and potential applications in enzyme replacement therapies, drug design, and metabolic engineering. This research not only enhances our understanding of gldA's biological significance but also opens new avenues for innovative treatments targeting metabolic disorders and oxidative stress-related diseases.
