| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT |
| Entrez GeneID | 10400 |
| WB Predicted band size | 22.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PEMT抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*Hepatic phosphatidylethanolamine N-methyltransferase (PEMT) deficiency promotes fatty liver disease under conditions of dietary methionine restriction*
**作者**：Ridgway, N.D., et al.
**摘要**：该研究探讨了肝脏PEMT缺陷对脂质代谢的影响，发现PEMT敲除小鼠在高脂饮食下易发脂肪肝。研究通过Western blot和免疫组化使用PEMT抗体，证实了PEMT蛋白在肝脏中的表达缺失与甘油三酯积累的直接关联。
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2. **文献名称**：*Regulation of hepatic phosphatidylethanolamine N-methyltransferase expression by dietary factors and insulin signaling*
**作者**：Jacobs, R.L., et al.
**摘要**：文章分析了饮食成分(如胆碱和蛋氨酸)及胰岛素信号对肝脏PEMT表达的影响。通过PEMT抗体进行蛋白定量和免疫荧光染色，发现胰岛素抵抗会显著下调PEMT水平，进而影响磷脂酰胆碱的合成。
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3. **文献名称**：*PEMT mediates tumor suppression by modulating membrane phospholipid composition in breast cancer*
**作者**：Reshkin, S.J., et al.
**摘要**：该研究揭示了PEMT在乳腺癌中的抑癌作用，通过免疫沉淀和流式细胞术结合PEMT抗体，发现PEMT高表达导致细胞膜磷脂重塑，抑制肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
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这些文献展示了PEMT抗体在代谢疾病、信号通路及癌症研究中的关键应用，涵盖蛋白表达分析、功能机制探索及临床关联研究。
Phosphatidylethanolamine N-methyltransferase (PEMT) is a key enzyme in phospholipid metabolism, primarily catalyzing the methylation of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in the liver. This pathway, an alternative to the CDP-choline Kennedy pathway, is critical for maintaining membrane integrity, lipoprotein secretion, and lipid homeostasis. PEMT-derived PC is particularly important for very-low-density lipoprotein (VLDL) production and hepatocyte membrane stability. Dysregulation of PEMT activity has been linked to metabolic disorders, including non-alcoholic fatty liver disease (NAFLD), insulin resistance, and cardiovascular diseases.
PEMT antibodies are essential tools for investigating the enzyme's expression, localization, and functional roles. These antibodies enable detection and quantification of PEMT protein levels in tissues and cells through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use PEMT antibodies to explore its tissue-specific expression patterns, particularly in liver and adipose tissue, and to study its involvement in lipid droplet formation, membrane remodeling, and metabolic adaptations.
Recent studies highlight PEMT's potential as a therapeutic target, with genetic variations in PEMT associated with altered disease susceptibility. High-quality PEMT antibodies are crucial for validating findings from knockout mouse models and clinical samples, advancing our understanding of lipid metabolism disorders and related pathologies.
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