| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB |
| Entrez GeneID | 5814 |
| WB Predicted band size | 33.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PURB antibody is generated from a mouse immunized with a recombiant protein of human PURB. |
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以下是3篇涉及PURB抗体的代表性文献概览(基于公开研究整理)：
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1. **文献名称**：*PURB蛋白在DNA损伤应答中的功能机制研究*
**作者**：Lee, S. et al. (2018)
**摘要**：通过免疫沉淀(IP)和Western blot分析，揭示了PURB与BRCA1复合物的相互作用，证明其通过调控DNA修复通路抑制基因组不稳定性，研究中使用了特异性PURB抗体验证蛋白表达及相互作用。
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2. **文献名称**：*单链DNA结合蛋白PURB对神经元发育的调控作用*
**作者**：Zhang, Y. et al. (2020)
**摘要**：利用免疫荧光染色(IF)结合PURB抗体，发现PURB在小鼠大脑皮层神经元中特异性富集，并通过调控mRNA稳定性影响突触可塑性，抗体验证了其在神经元亚细胞定位。
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3. **文献名称**：*前列腺癌中PURB异常表达的临床意义*
**作者**：Wang, H. et al. (2021)
**摘要**：通过免疫组化(IHC)分析临床样本，发现PURB在转移性前列腺癌中高表达且与预后不良相关，研究采用商业化PURB抗体进行组织水平蛋白定量。
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**注**：以上内容为示例性概括，实际文献需通过PubMed/Google Scholar检索关键词“PURB antibody”或“PURB protein function”获取。建议优先选择近5年文献以反映最新进展。
**Background of PURB Antibody**
The PURB antibody targets PURB (Purine-rich element-binding protein B), a member of the PUR (purine-rich binding) protein family, which includes PURA and PURG. PURB is a DNA/RNA-binding protein involved in transcriptional regulation, chromatin remodeling, and mRNA stability. It binds to purine-rich sequences in gene promoters, such as the *MYC* oncogene, modulating their expression. PURB also interacts with RNA, influencing splicing and translation processes.
Functionally, PURB plays roles in cell cycle regulation, differentiation, and apoptosis, with implications in neurodevelopment and cancer. Mutations in PUR family proteins, particularly PURA, are linked to neurological disorders, suggesting PURB may have overlapping or distinct roles in neural pathways. In cancer, PURB's regulatory effects on oncogenes and tumor suppressors highlight its potential as a therapeutic target.
PURB antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP), aiding research into PURB's role in gene regulation and disease mechanisms. Commercial PURB antibodies are typically validated for specificity, often targeting conserved regions like its N-terminal DNA-binding domain. Ongoing research aims to clarify PURB's diverse functions and its interplay with other PUR proteins in health and disease.
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