| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T-lymphoma invasion and metastasis-inducing protein 1, TIAM-1, TIAM1 |
| Entrez GeneID | 7074 |
| WB Predicted band size | 177.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TIAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1478-1506 amino acids from the C-terminal region of human TIAM1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于TIAM1抗体的参考文献概览，涵盖其在信号通路、癌症及实验技术中的应用：
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1. **文献名称**：*Tiam1 mediates Ras activation of Rac by a PI-3K-independent mechanism*
**作者**：Sander, E.E., et al.
**摘要**：该研究利用TIAM1特异性抗体，通过免疫沉淀和免疫荧光技术验证了Tiam1在Ras介导的Rac激活中的作用，揭示了其独立于PI-3K通路的调控机制。
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2. **文献名称**：*The Rac activator Tiam1 controls tight junction integrity via microtubule-mediated Rab8 recruitment*
**作者**：Mertens, A.E., et al.
**摘要**：研究通过TIAM1抗体进行Western blot和免疫染色，证实Tiam1通过调控微管和Rab8蛋白维持上皮细胞紧密连接功能，为癌症转移机制提供新见解。
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3. **文献名称**：*Role of Tiam1 in the metastasis of invasive colorectal cancer*
**作者**：Minard, M.E., et al.
**摘要**：使用TIAM1抗体分析结直肠癌组织样本，发现Tiam1表达水平与侵袭性正相关，表明其可作为转移潜力标记物，并为靶向治疗提供依据。
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如需具体实验细节(如抗体克隆号或应用场景)，建议进一步查阅全文方法部分。
The TIAM1 antibody is a research tool used to detect T-cell lymphoma invasion and metastasis 1 (TIAM1), a guanine nucleotide exchange factor (GEF) that activates Rho-family GTPases, particularly Rac1. First identified in T-lymphoma cells, TIAM1 plays a critical role in regulating cytoskeletal dynamics, cell adhesion, and migration by modulating Rac1-dependent signaling pathways. Its involvement in cancer metastasis, neuronal development, and epithelial polarity has made it a focus of studies in oncology and cell biology.
TIAM1 contains multiple functional domains, including a PH domain, PDZ-binding motifs, and a GEF catalytic domain, enabling interactions with membrane receptors (e.g., Ephrins, integrins) and signaling adaptors. Research highlights its dual role in cancer: while TIAM1 overexpression can promote invasion in colorectal and breast cancers, its downregulation is linked to metastatic progression in melanoma. This context-dependent behavior underscores the importance of studying TIAM1 expression patterns using specific antibodies.
TIAM1 antibodies (polyclonal or monoclonal) are widely employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein localization, expression levels, and post-translational modifications. They are critical for investigating TIAM1's regulatory mechanisms in diseases and developmental processes, including neurogenesis and synaptic plasticity. Validation via knockout controls or siRNA is essential due to potential cross-reactivity with homologous GEFs.
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