| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lysine-specific demethylase 6B, 11411-, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, KDM6B, JMJD3, KIAA0346 |
| Entrez GeneID | 23135 |
| WB Predicted band size | 176.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This KDM6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-195 amino acids from the N-terminal region of human KDM6B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与KDM6B(N-term)抗体相关的文献摘要，供参考：
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1. **"KDM6B regulates chromatin accessibility to orchestrate hematopoietic stem cell development"**
- **作者**: Yu, V.W.C. et al.
- **摘要**: 本研究利用KDM6B(N-term)抗体进行染色质免疫沉淀(ChIP)，揭示了KDM6B通过调控染色质可及性在造血干细胞分化中的关键作用，并证实该抗体在识别内源性KDM6B蛋白中的特异性。
2. **"Dynamic regulation of histone H3K27 methylation and demethylase KDM6B in early embryonic development"**
- **作者**: Smith, A.G. et al.
- **摘要**: 通过Western blot和免疫荧光实验(使用KDM6B N端抗体)，作者发现KDM6B在小鼠早期胚胎发育中动态调控H3K27me3水平，影响多能性基因的表达。
3. **"Targeting KDM6B-mediated histone demethylation in inflammatory diseases"**
- **作者**: Li, H. et al.
- **摘要**: 该研究通过免疫组织化学(IHC)和流式细胞术(使用KDM6B N-term抗体)，证明KDM6B在巨噬细胞炎症反应中通过去甲基化H3K27me3促进促炎因子表达，为治疗炎症提供了新靶点。
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以上文献均通过KDM6B(N-term)抗体验证了该蛋白在染色质调控、发育及疾病中的功能，涵盖ChIP、WB、IHC等实验方法。具体文献来源可进一步通过PubMed或Sci-Hub结合标题及作者检索获取。
The KDM6B (N-term) antibody is designed to specifically target the N-terminal region of lysine demethylase 6B (KDM6B), also known as JMJD3. KDM6B is a member of the Jumonji C (JmjC) domain-containing histone demethylase family, which catalyzes the removal of methyl groups from histone H3 lysine 27 (H3K27me2/me1), a post-translational modification associated with transcriptional repression. Unlike its homolog KDM6A (UTX), KDM6B is inducible and plays critical roles in developmental processes, cellular differentiation, immune responses, and cancer progression. It regulates gene expression by interacting with key transcription factors and chromatin modifiers, thereby influencing cell fate decisions and inflammatory pathways.
The N-terminal region of KDM6B contains regulatory sequences essential for protein-protein interactions and subcellular localization. Antibodies targeting this domain are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to study KDM6B expression, function, and epigenetic regulation. Researchers employ this antibody to investigate its involvement in diseases like leukemia, solid tumors, and inflammatory disorders, where aberrant KDM6B activity may drive pathological gene expression. Validated applications often include detecting endogenous KDM6B in human, mouse, and rat samples, with specificity confirmed via knockout controls. Its role in stem cell pluripotency and cellular senescence further underscores its relevance in regenerative medicine and aging research.
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