| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methylcytosine dioxygenase TET2, 11411n2, TET2, KIAA1546 |
| Entrez GeneID | 54790 |
| WB Predicted band size | 22.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TET2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-554 amino acids from the N-terminal region of human TET2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TET2 (N-term)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *TET2 Loss Leads to Increased Hematopoietic Stem Cell Self-Renewal and Myeloid Transformation*
**作者**: Ko M, et al.
**摘要**: 本研究利用TET2 (N-term)抗体通过Western blot和免疫组化分析，验证了TET2蛋白在造血干细胞中的表达缺失如何促进自我更新和白血病发生。抗体特异性通过敲除细胞系验证。
2. **文献名称**: *Impaired Hematopoiesis in Tet2-Deficient Mice*
**作者**: Cimmino L, et al.
**摘要**: 文章通过TET2 (N-term)抗体检测小鼠模型中TET2蛋白的表达模式，发现其缺失导致骨髓分化异常。抗体应用于免疫沉淀实验，证实TET2与其他表观调控因子的相互作用。
3. **文献名称**: *TET2 Regulates 5-Hydroxymethylcytosine Signature in Cancer*
**作者**: Huang Y, et al.
**摘要**: 研究使用TET2 (N-term)抗体进行ChIP-seq和免疫荧光分析，揭示了TET2在癌症中通过调控5hmC修饰维持基因组稳定性。抗体特异性经肽段竞争实验验证。
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这些文献展示了TET2 (N-term)抗体在蛋白表达分析、功能机制研究和表观遗传修饰检测中的关键应用。如需具体文章链接或补充信息，可进一步检索PubMed或期刊数据库。
The TET2 (N-term) antibody is a tool designed to detect the N-terminal region of Tet methylcytosine dioxygenase 2 (TET2), a critical enzyme in epigenetic regulation. TET2. encoded by the TET2 gene (HGNC: 25941), catalyzes the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), initiating DNA demethylation and modulating gene expression. It plays a vital role in hematopoietic stem cell differentiation, and mutations in TET2 are frequently linked to hematologic malignancies, such as myelodysplastic syndromes and acute myeloid leukemia.
The N-terminal region of TET2 contains conserved domains involved in protein-protein interactions and subcellular localization. Antibodies targeting this region (e.g., residues within the first 200 amino acids) are often used to distinguish full-length TET2 isoforms from truncated variants, which may arise from alternative splicing or mutation-driven loss of function. Such antibodies are valuable in Western blotting, immunofluorescence, and immunoprecipitation to study TET2 expression, stability, or interactions in cell lines, tissues, or primary samples. Specificity is typically validated using TET2-knockout controls. Research applications include investigating TET2's role in epigenetic dysregulation, hematopoietic disorders, and cancer progression. However, users should confirm antibody compatibility with their experimental models, as truncating mutations in certain cancers may delete the N-terminal epitope.
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